ArticleBMC plant biology2021
Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress.
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Cloning and Expression Studies of Osmotin-Like Protein Gene from Solanum nigrum in Escherichia coli.Molecular biotechnology · 2026Article
- Article
- An Aquaporin Gene (Bioengineering (Basel, Switzerland) · 2023Article
- Comparative Transcriptomics of Multi-Stress Responses inInternational journal of molecular sciences · 2023Article
- In silico analysis of NAC gene family in the mangrove plant Avicennia marina provides clues for adaptation to intertidal habitats.Plant molecular biology · 2023Article
- Genetic and molecular mechanisms underlying mangrove adaptations to intertidal environments.iScience · 2022Review
- Comprehensive analysis of the NAC transcription factor gene family inFrontiers in plant science · 2022Article
- Mechanism of cotton resistance to abiotic stress, and recent research advances in the osmoregulation related genes.Frontiers in plant science · 2022Review
- Genome-Wide Identification ofFrontiers in genetics · 2022Article
- Multi-omics analyses on Kandelia obovata reveal its response to transplanting and genetic differentiation among populations.BMC plant biology · 2021Article
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundLow temperature is a major abiotic stress that seriously limits mangrove productivity and distribution. Kandelia obovata is the most cold-resistance specie in mangrove plants, but little is known about the molecular mechanism underlying its resistance to cold. Osmotin is a key protein associated with abiotic and biotic stress response in plants but no information about this gene in K. obovata was reported.
resultsIn this study, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant K. obovata. The KoOsmotin protein was composed of 221 amino acids and showed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal signal peptide, which were common signatures to most osmotins and pathogenesis-related 5 proteins. The three-dimensional (3D) model of KoOsmotin, contained one α-helix and eleven β-strands, was formed by three characteristic domains. Database comparisons of the KoOsmotin showed the closest identity (55.75%) with the osmotin 34 from Theobroma cacao. The phylogenetic tree also revealed that the KoOsmotin was clustered in the branch of osmotin/OLP (osmotin-like protien). The KoOsmotin protein was proved to be localized to both the plasma membrane and cytoplasm by the subcellular localization analysis. Gene expression showed that the KoOsmotin was induced primarily and highly in the leaves of K. obovata, but less abundantly in stems and roots. The overexpressing of KoOsmotin conferred cold tolerance in Escherichia coli cells.
conclusionAs we known, this is the first study to explore the osmotin of K. obovata. Our study provided valuable clues for further exploring the function of KoOsmotin response to stress.
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