ArticleBMC microbiology2021
A metabolic and physiological design study of Pseudomonas putida KT2440 capable of anaerobic respiration.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- Sustainable production of glutaric acid in microbial cell factories: Current advances and future prospects.Synthetic and systems biotechnology · 2026Review
- Hemoglobin expression reduces aerobic bioconversion of guaiacol in Pseudomonas putida despite non-limiting oxygen.AMB Express · 2026Article
- Measuring bacterial oxygen consumption rate to probe metabolic signature and antimicrobial susceptibility.European biophysics journal : EBJ · 2026Article
- The TonB-Dependent Transport System Facilitates the Uptake of Inorganic Metal Mediators in Pseudomonas putida KT2440 in a Bioelectrochemical System.Microbial biotechnology · 2025Article
- Prophage-encoded chitinase gene supports growth of its bacterial host isolated from deep-sea sediments.The ISME journal · 2025Article
- Bacterial Communities From Two Freshwater Aquaculture Systems in Northern Germany.Environmental microbiology reports · 2024Article
- Systems biology of electrogenic Pseudomonas putida - multi-omics insights and metabolic engineering for enhanced 2-ketogluconate production.Microbial cell factories · 2024Article
- Unveiling six novel bacterial strains for fipronil and thiobencarb biodegradation: efficacy, metabolic pathways, and bioaugmentation potential in paddy soil.Frontiers in microbiology · 2024Article
- Supplementation with Amino Acid Sources Facilitates Fermentative Growth of Shewanella oneidensis MR-1 in Defined Media.Applied and environmental microbiology · 2023Article
- Screening of natural phenazine producers for electroactivity in bioelectrochemical systems.Microbial biotechnology · 2023Article
- Novel prokaryotic system employing previously unknown nucleic acids-based receptors.Microbial cell factories · 2022Article
- In vivo, in vitro and in silico: an open space for the development of microbe-based applications of synthetic biology.Microbial biotechnology · 2022Article
- Bridging the gap between microbial limits and extremes in space: space microbial biotechnology in the next 15 years.Microbial biotechnology · 2022Article
- Micro-aerobic production of isobutanol with engineeredEngineering in life sciences · 2021Article
- The anoxic electrode-driven fructose catabolism of Pseudomonas putida KT2440.Microbial biotechnology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPseudomonas putida KT2440 is a metabolically versatile, HV1-certified, genetically accessible, and thus interesting microbial chassis for biotechnological applications. However, its obligate aerobic nature hampers production of oxygen sensitive products and drives up costs in large scale fermentation. The inability to perform anaerobic fermentation has been attributed to insufficient ATP production and an inability to produce pyrimidines under these conditions. Addressing these bottlenecks enabled growth under micro-oxic conditions but does not lead to growth or survival under anoxic conditions.
resultsHere, a data-driven approach was used to develop a rational design for a P. putida KT2440 derivative strain capable of anaerobic respiration. To come to the design, data derived from a genome comparison of 1628 Pseudomonas strains was combined with genome-scale metabolic modelling simulations and a transcriptome dataset of 47 samples representing 14 environmental conditions from the facultative anaerobe Pseudomonas aeruginosa.
conclusionsThe results indicate that the implementation of anaerobic respiration in P. putida KT2440 would require at least 49 additional genes of known function, at least 8 genes encoding proteins of unknown function, and 3 externally added vitamins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.