Evidence map›Paper›PMID 33405522›Full record

ArticleACS biomaterials science & engineering2019

Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts.

Jenna N Harvestine, Clay S Sheaff, Cai Li, Anne K Haudenschild, Marissa A Gionet-Gonzales, Jerry C Hu, Kyriacos A Athanasiou, Laura Marcu, J Kent Leach

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS biomaterials science & engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jenna N HarvestineDepartment of Biomedical Engineering, University of California, Davis, Davis, California 95616, United States.
Clay S SheaffDepartment of Biomedical Engineering, University of California, Davis, Davis, California 95616, United States.
Cai LiDepartment of Biomedical Engineering, University of California, Davis, Davis, California 95616, United States.
Anne K HaudenschildDepartment of Biomedical Engineering, University of California, Davis, Davis, California 95616, United States.
Marissa A Gionet-GonzalesDepartment of Biomedical Engineering, University of California, Davis, Davis, California 95616, United States.
Jerry C HuDepartment of Biomedical Engineering, University of California, Irvine, Irvine California 92697, United States.
Kyriacos A AthanasiouDepartment of Biomedical Engineering, University of California, Irvine, Irvine California 92697, United States.
Laura MarcuDepartment of Biomedical Engineering, University of California, Davis, Davis, California 95616, United States.
J Kent LeachDepartment of Biomedical Engineering, University of California, Davis, Davis, California 95616, United States.ORCID http://orcid.org/0000-0002-1673-3335
University of California, Davis · USUniversity of California, Irvine · US

Funding

Dual peptide presentation from bioengineered carriers to potentiate stromal cell function and tissue repairR01DE025899 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2017 to 2021
$2.5M
Biomaterial regulation of cell spheroids to synergistically enhance bone healingR01DE025475 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2015 to 2019
$1.9M
NIDCR NIH HHS R01 DE025475NIDCR NIH HHS R01 DE025899
6 · The paper itself

Abstract

There is a critical need to develop noninvasive, nondestructive methods for assessing the quality of engineered constructs prior to implantation. Currently, the composition and maturity of engineered tissues are assessed using destructive, costly, and time-consuming biochemical and mechanical analyses. The goal of this study was to use noninvasive, multimodal imaging to monitor osteogenic differentiation and matrix deposition by human mesenchymal stem/stromal cells (MSCs) during in vitro culture. MSCs were encapsulated in alginate hydrogels and cultured in osteogenic conditions for 4 weeks. Samples were evaluated using fluorescence lifetime imaging (FLIm) and ultrasound backscatter microscopy (UBM) prior to traditional biochemical and mechanical testing. Using linear regression analysis, we identified strong correlations between imaging parameters (e.g., fluorescence lifetime and acoustic attenuation coefficient) and destructive mechanical and biochemical tests to assess the maturation of osteogenically induced constructs. These data demonstrate the promise of nondestructive label-free imaging techniques to noninvasively ascertain the progression and maturity of tissue engineered bone grafts.

Indexed as

fluorescence lifetime imagingmesenchymal stem/stromal cellosteogenic differentiationosteogenic graftultrasound backscatter

Identifiers

PMID33405522
PMCPMC8594456
OpenAlexW2924149280

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.