ArticleMethods in molecular biology (Clifton, N.J.)2021
Prediction of Cellular Burden with Host-Circuit Models.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Disentangling Metabolite Detection from Pathway Stress Using Biosensors.ACS synthetic biology · 2025Article
- Engineering plasmid copy number heterogeneity for dynamic microbial adaptation.Nature microbiology · 2024Article
- Accelerating Genetic Sensor Development, Scale-up, and Deployment Using Synthetic Biology.Biodesign research · 2024Article
- Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation.Microbial cell factories · 2022Article
- Understanding and mathematical modelling of cellular resource allocation in microorganisms: a comparative synthesis.BMC bioinformatics · 2021Review
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Authors and funding
3 authors.
Funding
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Abstract
Heterologous gene expression draws resources from host cells. These resources include vital components to sustain growth and replication, and the resulting cellular burden is a widely recognized bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host-circuit models to predict the impact of design parameters on both burden and circuit functionality. Our approach relies on a new generation of computational models for microbial growth that can flexibly accommodate resource bottlenecks encountered in gene circuit design. Adoption of this modeling paradigm can facilitate fast and robust design cycles in synthetic biology.
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Registered trials
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