ArticleMethods in molecular biology (Clifton, N.J.)2021
Computer-Aided Design and Pre-validation of Large Batches of DNA Assemblies.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- InSillyClo, a User-Friendly Web Application to Assist Large-Scale Golden Gate Cloning and MoClo Workflows.ACS synthetic biology · 2026Article
- Modular DNA Construct Design for High-Throughput Golden Gate Assembly.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Biofoundry-Scale DNA Assembly Validation Using Cost-Effective High-Throughput Long-Read Sequencing.ACS synthetic biology · 2024Article
- Standardization of Synthetic Biology Tools and Assembly Methods forACS synthetic biology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type-2S restriction enzymes allow the routine assembly of large batches of synthetic constructs from individual genetic parts. However, design flaws in the part sequence can cause assembly failures, incurring troubleshooting costs and project delays. As a result, the careful design and checking of the assembly plan is often a bottleneck of large assembly projects, and may require computational support. This chapter demonstrates the use of two free and open-source web applications accelerating this task by automating genetic part design and simulating type-2S cloning to detect potential assembly issues.
Indexed as
Identifiers
33405220What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.