Evidence map›Paper›PMID 33404779›Full record

ArticleMikrochimica acta2021

Portable electrochemical immunosensor for detection of Mycobacterium tuberculosis secreted protein CFP10-ESAT6 in clinical sputum samples.

Umi Zulaikha Mohd Azmi, Nor Azah Yusof, Jaafar Abdullah, Shahrul Ainliah Alang Ahmad, Fatin Nabilah Mohd Faudzi, Nurul Hanun Ahmad Raston, Siti Suraiya, Poh Shing Ong, Devandran Krishnan, Nur Khairunnisa Sahar

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Review
  6. Biosensors; nanomaterial-based methods in diagnosing ofJournal of clinical tuberculosis and other mycobacterial diseases · 2024
    Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Aptasensor for the Detection ofNanomaterials (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Umi Zulaikha Mohd AzmiInstitute of Advanced Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. umizulaikha.ika@gmail.com.ORCID 0000-0002-0525-518X
Nor Azah YusofInstitute of Advanced Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. azahy@upm.edu.my.
Jaafar AbdullahInstitute of Advanced Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Shahrul Ainliah Alang AhmadInstitute of Advanced Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Fatin Nabilah Mohd FaudziInstitute of Advanced Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Nurul Hanun Ahmad RastonSchool of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
Siti SuraiyaSchool of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
Poh Shing OngNanoMalaysia Berhad, a CLG under the Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), 50450, Kuala Lumpur, Malaysia.
Devandran KrishnanNanoMalaysia Berhad, a CLG under the Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), 50450, Kuala Lumpur, Malaysia.
Nur Khairunnisa SaharSchool of Medicine, Trinity Biomedical Sciences Institute, Trinity College, Dublin 2, Ireland.

Funding

Nanomalaysia Berhad P17014Universiti Putra Malaysia 9528600Universiti Putra Malaysia UPM/800-4/11/MRUN/2018/5539230
6 · The paper itself

Abstract

An early detection of Mycobacterium tuberculosis is very important to reduce the number of fatal cases and allow for fast recovery. However, the interpretation of the result from smear microscopy requires skilled personnel due to the propensity of the method to produce false-negative results. In this work, a portable, rapid, and simple sandwich-type immunosensor reader has been developed that is able to detect the presence of M. tuberculosis in sputum samples. By using sandwich-type immunosensor, an anti-CFP10-ESAT6 antibody was immobilized onto the graphene/polyaniline (GP/PANI)-modified gold screen-printed electrode. After incubation with the target CFP10-ESAT6 antigen, the iron/gold magnetic nanoparticles (Fe

Indexed as

Bacterial ProteinsElectrochemical TechniquesGoldHumansImmunoassayLimit of DetectionMagnetite NanoparticlesMycobacterium tuberculosisRecombinant Fusion ProteinsReproducibility of ResultsSensitivity and SpecificitySputumTuberculosisBacterial ProteinsESAT-6-CFP10 fusion proteinGoldMagnetite NanoparticlesRecombinant Fusion ProteinsAntigenic target (CFP10-ESAT6)Clinical studyDifferential pulse voltammetryPortable readerSandwich-type immunosensorScreen-printed electrodeSputum analysis

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.