Evidence map›Paper›PMID 33403543›Full record

ArticleCanadian journal of anaesthesia = Journal canadien d'anesthesie2021

Untargeted metabolomics profiling of skeletal muscle samples from malignant hyperthermia susceptible patients.

Barbara Bojko, Tijana Vasiljevic, Ezel Boyaci, Anna Roszkowska, Natalia Kraeva, Carlos A Ibarra Moreno, Annabel Koivu, Marcin Wąsowicz, Amy Hanna, Susan Hamilton and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Canadian journal of anaesthesia = Journal canadien d'anesthesie, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Skeletal muscle disorders as risk factors for type 2 diabetes.Molecular and cellular endocrinology · 2025
    Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. ACS environmental Au · 2022
    Review
  10. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 4 countries.

Barbara Bojko *Department of Chemistry, University of Waterloo, Waterloo, ON, Canada.
Tijana Vasiljevic *Department of Chemistry, University of Waterloo, Waterloo, ON, Canada.
Ezel BoyaciDepartment of Chemistry, University of Waterloo, Waterloo, ON, Canada.
Anna RoszkowskaDepartment of Chemistry, University of Waterloo, Waterloo, ON, Canada.
Natalia KraevaMalignant Hyperthermia Investigation Unit, Department of Anesthesia, University Health Network, University of Toronto, 323-200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada.
Carlos A Ibarra MorenoMalignant Hyperthermia Investigation Unit, Department of Anesthesia, University Health Network, University of Toronto, 323-200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada.
Annabel KoivuMalignant Hyperthermia Investigation Unit, Department of Anesthesia, University Health Network, University of Toronto, 323-200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada.
Marcin WąsowiczMalignant Hyperthermia Investigation Unit, Department of Anesthesia, University Health Network, University of Toronto, 323-200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada.
Amy HannaDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, USA.
Susan HamiltonDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, USA.
Sheila RiaziMalignant Hyperthermia Investigation Unit, Department of Anesthesia, University Health Network, University of Toronto, 323-200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada. Sheila.Riazi@uhn.ca.ORCID http://orcid.org/0000-0002-0670-1516
Janusz PawliszynDepartment of Chemistry, University of Waterloo, Waterloo, ON, Canada.
University Health Network · CAUniversity of Waterloo · CABaylor College of Medicine · US

Funding

The HH: A Large Cohort of Patients with Congenital Myopathies of Uncertain EtiologyR01AR071381 · NIAMS · RUSH UNIVERSITY MEDICAL CENTER · PI FILL, MICHAEL, RIAZI, SHEILA · 2017 to 2021
$2.7M
NIAMS NIH HHS R01 AR071381
6 · The paper itself

Abstract

purposeMalignant hyperthermia (MH) is a potentially fatal hypermetabolic condition triggered by certain anesthetics and caused by defective calcium homeostasis in skeletal muscle cells. Recent evidence has revealed impairment of various biochemical pathways in MH-susceptible patients in the absence of anesthetics. We hypothesized that clinical differences between MH-susceptible and control individuals are reflected in measurable differences in myoplasmic metabolites.

methodsWe performed metabolomic profiling of skeletal muscle samples from MH-negative (control) individuals and MH-susceptible patients undergoing muscle biopsy for diagnosis of MH susceptibility. Cellular metabolites were extracted from 33 fresh and 87 frozen human muscle samples using solid phase microextraction and Metabolon® untargeted biochemical profiling platforms, respectively. Ultra-performance liquid chromatography-high resolution mass spectrometry was used for metabolite identification and validation, followed by analysis of differences in metabolites between the MH-susceptible and MH-negative groups.

resultsSignificant fold-change differences between the MH-susceptible and control groups in metabolites from various pathways were found (P value range: 0.009 to < 0.001). These included accumulation of long chain acylcarnitines, diacylglycerols, phosphoenolpyruvate, histidine pathway metabolites, lysophosphatidylcholine, oxidative stress markers, and phosphoinositols, as well as decreased levels of monoacylglycerols. The results from both analytical platforms were in agreement.

conclusionThis metabolomics study indicates a shift from utilization of carbohydrates towards lipids for energy production in MH-susceptible individuals. This shift may result in inefficiency of beta-oxidation, and increased muscle protein turnover, oxidative stress, and/or lysophosphatidylcholine levels.

Indexed as

HalothaneMalignant HyperthermiaHumansHyperthermiaMetabolomicsMuscle, SkeletalHalothaneMalignant hyperthermiaMetabolomics

Identifiers

PMID33403543
PMCPMC8185566
OpenAlexW3118841793

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.