ArticleACS omega2020
Aptamer-Based Lateral Flow Biosensor for Rapid Detection of Salivary Cortisol.
Article in ACS omega, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 140 citations in OpenAlex.
- Sensitive detection of unopposed estrogen using estrogen receptor functionalized nanoprobes for cancer diagnosis.Discover nano · 2026Review
- Ultrasensitive de novo Protein Sandwich Lateral flow Fluorescence Assay for Cortisol in Saliva and Serum using Quantum dots and Europium Nanoparticles.Journal of fluorescence · 2026Article
- Structure-enhanced deep learning accelerates aptamer selection for small molecule families like steroids.Briefings in bioinformatics · 2025Article
- Picogram-level detection of endotoxin in human saliva using ultrasensitive surface enhanced Raman scattering in lateral flow assay devices.RSC advances · 2025Article
- Article
- POC Sensor Systems and Artificial Intelligence-Where We Are Now and Where We Are Going?Biosensors · 2025Review
- Development of Novel ssDNA Aptamers for Detection of Receptor-Binding Domain of SARS-COV‑2.ACS omega · 2025Article
- Competitive enzyme linked aptamer based assay for salivary melatonin detection.Scientific reports · 2025Article
- Adding a Twist to Lateral Flow Immunoassays: A Direct Replacement of Antibodies with Helical Affibodies, from Selection to Application.Journal of the American Chemical Society · 2025Article
- Electrochemical Biosensors by Means of Molecularly Imprinted Polymers (MIPs) Cortisol Recognition.Polymers · 2025Article
- Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test.Biosensors · 2025Article
- Cyclodextrin Inclusion Complexes with Hydrocortisone-Type Corticosteroids.Pharmaceutics · 2024Review
- Enzyme-free immunoassay for rapid, sensitive, and selective detection of C-reactive protein.Analytical and bioanalytical chemistry · 2024Article
- A double-conducting polymer nanowire-based electrochemical aptasensor for highly sensitive and low fouling detection of cortisol in saliva.Mikrochimica acta · 2024Article
- Bidirectional hybridized hairpin DNA fluorescent aptasensor based on Au-Pd NPs and CDs for ratiometric detection of AFB1.Mikrochimica acta · 2024Article
- A Gold Nanoparticle-Based Cortisol Aptasensor for Non-Invasive Detection of Fish Stress.Biomolecules · 2024Article
- Net-Shaped DNA Nanostructure-Based Lateral Flow Assays for Rapid and Sensitive SARS-CoV-2 Detection.Analytical chemistry · 2024Article
- Ultrasensitive electrochemical sensor for detection of salivary cortisol in stress conditions.Mikrochimica acta · 2024Article
- Ionic liquid-caged nucleic acids enable active folding-based molecular recognition with hydrolysis resistance.Nucleic acids research · 2024Article
- The Use of Wearable Technology in Providing Assistive Solutions for Mental Well-Being.Sensors (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a disposable point-of-care (POC) aptamer-based biosensor for the detection of salivary cortisol. Nonstressful and noninvasive sampling of saliva compared to that of blood makes saliva an attractive biological matrix in developing POC devices for biomarker monitoring. Aptamers are attractive as recognition elements for multiple reasons, including their specific chemical synthesis, high stability, lack of immunogenicity, and cell-free evolution. A duplex aptamer conjugated to the surface of Au nanoparticles (AuNPs) by Au-S bonds is utilized as the sensor probe in a lateral flow assay (LFA) device. The addition of saliva samples containing cortisol makes the cortisol-aptamer undergo conformational changes and dissociate from the capture probe. Increasing cortisol concentration in the dispensed saliva sample results in increased dissociation and leads to increased binding of AuNP conjugate on the test line. Therefore, the color intensity of the test line on the LFA is a direct function of the concentration of cortisol in saliva. This simple and fast method provides detection in the cortisol range of ∼0.5-15 ng/mL, which is in the clinically accepted range for salivary cortisol. The limit of detection was 0.37 ng/mL, and the accuracy was confirmed by enzyme-linked immunosorbent assay (ELISA) testing results. High selectivity was observed for salivary cortisol against other closely related steroids and stress biomarkers present in saliva.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.