ArticleMolecules (Basel, Switzerland)2021
Immobilization of Eversa
Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 54 citations in OpenAlex.
- Enzymatic Conversion of Mixed Neem (ACS omega · 2025Article
- Enzymatic Hydroesterification of Waste Oils: Combilipase-Assisted Ethyl Ester Synthesis via a Dual-Step Biocatalytic Process.ACS omega · 2025Article
- Strategies for the Transformation of Waste Cooking Oils into High-Value Products: A Critical Review.Polymers · 2025Review
- Extending the computational and experimental analysis of lipase active site selectivity.Bioprocess and biosystems engineering · 2024Article
- Enzyme Immobilization.Molecules (Basel, Switzerland) · 2023Article
- Fatty acid wax from epoxidation and hydrolysis treatments of waste cooking oil: synthesis and properties.RSC advances · 2022Article
- Immobilized Forms of theJournal of fungi (Basel, Switzerland) · 2021Article
- Carrier-Free Immobilization of α-Galactosidase as Nano-Biocatalysts for Synthesizing Prebiotic α-Galacto-Oligosaccharides.Molecules (Basel, Switzerland) · 2021Article
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
The performance of the previously optimized magnetic cross-linked enzyme aggregate of Eversa (Eversa-mCLEA) in the enzymatic synthesis of biolubricants by transesterification of waste cooking oil (WCO) with different alcohols has been evaluated. Eversa-mCLEA showed good activities using these alcohols, reaching a transesterification activity with isoamyl alcohol around 10-fold higher than with methanol. Yields of isoamyl fatty acid ester synthesis were similar using WCO or refined oil, confirming that this biocatalyst could be utilized to transform this residue into a valuable product. The effects of WCO/isoamyl alcohol molar ratio and enzyme load on the synthesis of biolubricant were also investigated. A maximum yield of around 90 wt.% was reached after 72 h of reaction using an enzyme load of 12 esterification units/g oil and a WCO/alcohol molar ratio of 1:6 in a solvent-free system. At the same conditions, the liquid Eversa yielded a maximum ester yield of only 34%. This study demonstrated the great changes in the enzyme properties that can be derived from a proper immobilization system. Moreover, it also shows the potential of WCO as a feedstock for the production of isoamyl fatty acid esters, which are potential candidates as biolubricants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.