Evidence map›Paper›PMID 33401455›Full record

ArticleInternational journal of molecular sciences2021

First Report of CRISPR/Cas9 Mediated DNA-Free Editing of

Sapna Badhan, Andrew S Ball, Nitin Mantri

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed, 3 pooled it
10.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed, 3 syntheses or guidelines pooled it, 182 citations in OpenAlex.

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  19. An overview of heat stress in Chickpea (Molecular breeding : new strategies in plant improvement · 2025
    Review
  20. Genome editing in grain legumes for food security.Frontiers in genome editing · 2025
    Review

9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Sapna BadhanThe Pangenomics Lab, School of Science, RMIT University, Melbourne 3000, Australia.
Andrew S BallThe Pangenomics Lab, School of Science, RMIT University, Melbourne 3000, Australia.ORCID 0000-0003-2387-968X
Nitin MantriThe Pangenomics Lab, School of Science, RMIT University, Melbourne 3000, Australia.ORCID 0000-0002-7621-035X
RMIT University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The current genome editing system Clustered Regularly Interspaced Short Palindromic Repeats Cas9 (CRISPR/Cas9) has already confirmed its proficiency, adaptability, and simplicity in several plant-based applications. Together with the availability of a vast amount of genome data and transcriptome data, CRISPR/Cas9 presents a massive opportunity for plant breeders and researchers. The successful delivery of ribonucleoproteins (RNPs), which are composed of Cas9 enzyme and a synthetically designed single guide RNA (sgRNA) and are used in combination with various transformation methods or lately available novel nanoparticle-based delivery approaches, allows targeted mutagenesis in plants species. Even though this editing technique is limitless, it has still not been employed in many plant species to date. Chickpea is the second most crucial winter grain crop cultivated worldwide; there are currently no reports on CRISPR/Cas9 gene editing in chickpea. Here, we selected the

Indexed as

CRISPR-Associated Protein 9Stress, PhysiologicalCicerCoenzyme A LigasesDroughtsGene EditingGene Knockout TechniquesLigninPlant ProteinsPlants, Genetically ModifiedTranscription Factors4-coumarate-CoA ligaseCoenzyme A LigasesCRISPR-Associated Protein 9LigninPlant ProteinsTranscription Factors4-coumarate ligase (4CL)chickpeaCRISPR/Cas9DNA free gene editingdrought stressprotoplastReveille 7 (RVE7)

Identifiers

PMID33401455
PMCPMC7795094
OpenAlexW3115890091

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.