ArticleNature biotechnology2021
Left-handed DNA-PAINT for improved super-resolution imaging in the nucleus.
Article in Nature biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes.Nano letters · 2026Article
- Optically switchable CLEAR probes enable rapid, biocompatible and high-efficiency fluorophore exchange for ultra-plex, high-resolution immunofluorescence imaging.Chemical science · 2026Article
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells.bioRxiv : the preprint server for biology · 2026Article
- Left-Handed Helices in DNA Nanotechnology.JACS Au · 2026Review
- On Degradation-Induced Leak in Heterochiral DNA Strand Displacement Cascades.ACS synthetic biology · 2026Article
- Utilizing the predictable binding kinetics of DNA-PAINT to denoise super-resolution images.Nature communications · 2026Article
- 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination.Nature methods · 2026Article
- Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures.The EMBO journal · 2025Article
- Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution.Nature communications · 2025Article
- From Biophysics to Biomedical Physics.ACS bio & med chem Au · 2025Review
- Microscopy methods for the in vivo study of nanoscale nuclear organization.Biochemical Society transactions · 2025Review
- Reduced Non-Specific Binding of Super-Resolution DNA-PAINT Markers by Shielded DNA-PAINT Labeling Protocols.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Article
- Quantitative DNA-PAINT imaging of AMPA receptors in live neurons.Cell reports methods · 2023Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (International ed. in English) · 2022Article
- Picasso-server: a community-based, open-source processing framework for super-resolution data.Communications biology · 2022Article
- DNA Walkers for Biosensing Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Technological advances in super-resolution microscopy to study cellular processes.Molecular cell · 2022Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA point accumulation in nanoscale topography (DNA-PAINT) increases the resolution and multiplexing capabilities of super-resolution imaging, but cellular DNA interferes with DNA-DNA hybridization between target and probe in the nucleus. Here, we introduce left-handed DNA (L-DNA) oligomers that do not hybridize to natural right-handed DNA (R-DNA) and demonstrate that L-DNA-PAINT has the same specificity and multiplexing capability as R-DNA-PAINT, but improves the imaging of nuclear targets by substantially reducing background signal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.