ArticleCommunications biology2021
Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Molecular basis of NNature communications · 2026Article
- Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondria.Plant molecular biology · 2026Article
- A high-throughput heterologous expression platform for plant synthetic biology based on Arabidopsis suspension cells.Journal of experimental botany · 2026Article
- Mechanistic insights into nitrogen fertilizer regulation of carbon-nitrogen cycling and greenhouse gas emissions: a metagenomics-based investigation.Frontiers in microbiology · 2026Article
- Article
- Computationally Guided Design of a Soluble, Abundant and Functional AnfH Variant for the Expression of Fe-Only Nitrogenase in Plant Mitochondria.Plant biotechnology journal · 2025Article
- Iron-molybdenum cofactor synthesis by a thermophilic nitrogenase devoid of the scaffold NifEN.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Graphene promotes the growth of Vigna angularis by regulating the nitrogen metabolism and photosynthesis.PloS one · 2024Article
- Regulatory response to a hybrid ancestral nitrogenase inMicrobiology spectrum · 2023Article
- Organelle-dependent polyprotein designs enable stoichiometric expression of nitrogen fixation components targeted to mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The structural components of the Azotobacter vinelandii iron-only nitrogenase, AnfDKG, form a protein complex within the plant mitochondrial matrix.Plant molecular biology · 2023Article
- Dancing to a different tune, can we switch from chemical to biological nitrogen fixation for sustainable food security?PLoS biology · 2023Article
- Ammonia Production Using Bacteria and Yeast toward a Sustainable Society.Bioengineering (Basel, Switzerland) · 2023Review
- Editorial: The impact of climate change on nutrient composition of staple foods and the role of diversification in increasing food system resilience.Frontiers in plant science · 2023Article
- Functional expression of the nitrogenase Fe protein in transgenic rice.Communications biology · 2022Article
- Nitrogenase Cofactor Maturase NifB Isolated from Transgenic Rice is Active in FeMo-co Synthesis.ACS synthetic biology · 2022Article
- Environmental Galenics: large-scale fortification of extant microbiomes with engineered bioremediation agents.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Article
- Biological nitrogen fixation and prospects for ecological intensification in cereal-based cropping systems.Field crops research · 2022Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineering nitrogen fixation in eukaryotes requires high expression of functional nitrogenase structural proteins, a goal that has not yet been achieved. Here we build a knowledge-based library containing 32 nitrogenase nifH sequences from prokaryotes of diverse ecological niches and metabolic features and combine with rapid screening in tobacco to identify superior NifH variants for plant mitochondria expression. Three NifH variants outperform in tobacco mitochondria and are further tested in yeast. Hydrogenobacter thermophilus (Aquificae) NifH is isolated in large quantities from yeast mitochondria and fulfills NifH protein requirements for efficient N
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