Evidence map›Paper›PMID 33397995›Full record

ArticleCommunications biology2021

Single-cell fluidic force microscopy reveals stress-dependent molecular interactions in yeast mating.

Marion Mathelié-Guinlet, Felipe Viela, Jérôme Dehullu, Sviatlana Filimonava, Jason M Rauceo, Peter N Lipke, Yves F Dufrêne

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Marion Mathelié-GuinletLouvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte L7.07.07, 1348, Louvain-la-Neuve, Belgium.ORCID 0000-0001-9023-3470
Felipe VielaLouvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte L7.07.07, 1348, Louvain-la-Neuve, Belgium.ORCID 0000-0003-0566-9291
Jérôme DehulluLouvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte L7.07.07, 1348, Louvain-la-Neuve, Belgium.
Sviatlana FilimonavaDepartment of Sciences, John Jay College of the City University of New York, New York, NY, 10019, USA.
Jason M RauceoDepartment of Sciences, John Jay College of the City University of New York, New York, NY, 10019, USA.
Peter N LipkeBiology Department, Brooklyn College of the City University of New York, 2900 Bedford Avenue, Brooklyn, NY, 11210, USA. PLipke@brooklyn.cuny.edu.ORCID 0000-0003-1733-0690
Yves F DufrêneLouvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte L7.07.07, 1348, Louvain-la-Neuve, Belgium. yves.dufrene@uclouvain.be.ORCID 0000-0002-7289-4248
UCLouvain · BECity University of New York · US

Funding

Pathogenic Yeast Stress Signaling NetworksSC3GM111133 · NIGMS · JOHN JAY COLLEGE OF CRIMINAL JUSTICE · PI RAUCEO, JASON MALCOLM · 2015 to 2018
$471k
NIGMS NIH HHS SC3 GM111133
6 · The paper itself

Abstract

Sexual agglutinins of the budding yeast Saccharomyces cerevisiae are proteins mediating cell aggregation during mating. Complementary agglutinins expressed by cells of opposite mating types "a" and "α" bind together to promote agglutination and facilitate fusion of haploid cells. By means of an innovative single-cell manipulation assay combining fluidic force microscopy with force spectroscopy, we unravel the strength of single specific bonds between a- and α-agglutinins (~100 pN) which require pheromone induction. Prolonged cell-cell contact strongly increases adhesion between mating cells, likely resulting from an increased expression of agglutinins. In addition, we highlight the critical role of disulfide bonds of the a-agglutinin and of histidine residue H

Indexed as

Mating FactorSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsStress, Mechanicala-agglutinin protein, S cerevisiaeMating FactorSaccharomyces cerevisiae Proteins

Identifiers

PMID33397995
PMCPMC7782832
OpenAlexW3120761783

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.