Evidence map›Paper›PMID 33397829›Full record

ArticleJournal of microbiology and biotechnology2021

Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets.

Changwoo Park, Jina Lee, Zohaib Ul Hassan, Keun Bon Ku, Seong-Jun Kim, Hong Gi Kim, Edmond Changkyun Park, Gun-Soo Park, Daeui Park, Seung-Hwa Baek and 7 more

Open access · bronzeAbstract read
In one paragraph

Article in Journal of microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed, 1 pooled it
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.

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  13. Establishing methods to monitor H5N1 influenza virus in dairy cattle milk.medRxiv : the preprint server for health sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 8 institutions in 1 country.

Changwoo ParkMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Jina LeeMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Zohaib Ul HassanMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Keun Bon KuCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Seong-Jun KimCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Hong Gi KimCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Edmond Changkyun ParkCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Gun-Soo ParkCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Daeui ParkCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Seung-Hwa BaekCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Dongju ParkMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Jihye LeeZoonotic Virus Laboratory, Institut Pasteur Korea, Seongnam-si, Gyeonggi-do 13488, Republic of Korea.
Sangeun JeonZoonotic Virus Laboratory, Institut Pasteur Korea, Seongnam-si, Gyeonggi-do 13488, Republic of Korea.
Seungtaek KimZoonotic Virus Laboratory, Institut Pasteur Korea, Seongnam-si, Gyeonggi-do 13488, Republic of Korea.
Chang-Seop LeeDepartment of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986, Republic of Korea.
Hee Min YooMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Seil KimMicrobiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Korea Research Institute of Chemical Technology · KRKorea Research Institute of Standards and Science · KRChungnam National University · KRInstitut Pasteur Korea · KRJeonbuk National University Hospital · KRKorea Basic Science Institute · KRKorea Food Research Institute · KRKorea Institute of Toxicology · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The World Health Organization (WHO) has declared the coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method, which is the gold standard test that involves the amplification of viral RNA. However, the RT-qPCR assay has limitations in terms of sensitivity and quantification. In this study, we tested both qPCR and droplet digital PCR (ddPCR) to detect low amounts of viral RNA. The cycle threshold (C

Indexed as

AnimalsCell LineChlorocebus aethiopsCOVID-19Gene DosageHumansNucleic Acid ProbesReal-Time Polymerase Chain ReactionRNA, ViralSARS-CoV-2Sensitivity and SpecificityVero CellsNucleic Acid ProbesRNA, ViralCOVID-19droplet digital PCR (ddPCR)envelope protein genenucleocapsid protein genereverse transcription-quantitative polymerase chain reaction (RT-qPCR)SARS-CoV-2

Identifiers

PMID33397829
PMCPMC9705847
OpenAlexW3120872027

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.