Evidence map›Paper›PMID 33397204›Full record

ArticleStem cells and development2021

microRNA Sequencing of CD34+ Sorted Adipose Stem Cells Undergoing Endotheliogenesis.

Shahensha Shaik, Elizabeth Martin, Daniel Hayes, Jeffrey Gimble, Ram Devireddy

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Shahensha ShaikBioengineering Laboratory, Department of Mechanical Engineering and Louisiana State University, Baton Rouge, Louisiana, USA.
Elizabeth MartinDepartment of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, Louisiana, USA.
Daniel HayesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, Pennsylvania, USA.
Jeffrey GimbleLa Cell, LLC and Obatala Sciences, Inc., New Orleans, Louisiana, USA.
Ram DevireddyBioengineering Laboratory, Department of Mechanical Engineering and Louisiana State University, Baton Rouge, Louisiana, USA.
Louisiana State University · USPennsylvania State University · US

Funding

Spatiotemporal Modulation of Osteogenesis in a 3-D Stromal/Stem Cell ModelR01DE024790 · NIDCR · PENNSYLVANIA STATE UNIVERSITY, THE · PI HAYES, DANIEL J. · 2015 to 2019
$1.7M
Assessing a Novel Paradigm of Inducing CryotoleranceR21DK091852 · NIDDK · LOUISIANA STATE UNIV A&M COL BATON ROUGE · PI DEVIREDDY, RAM · 2012 to 2013
$364k
NIDCR NIH HHS R01 DE024790NIDDK NIH HHS R21 DK091852
6 · The paper itself

Abstract

While several microRNAs (miRNAs) that regulate the endotheliogenesis and further promote angiogenesis have been identified in various cancers, the identification of miRNAs that can drive the differentiation of adipose derived stromal/stem cells (ASCs) into the endothelial lineage has been largely unexplored. In this study, CD34+ ASCs sorted using magnetic bead separation were induced to differentiate along the endothelial pathway. miRNA sequencing of ASCs at day 3, 9, and 14 of endothelial differentiation was performed on Ion Proton sequencing system. The data obtained by this high-throughput method were aligned to the human genome HG38, and the differentially expressed miRNAs during endothelial differentiation at various time points (day 3, 9, and 14) were identified. The gene targets of the identified miRNAs were obtained through miRWalk database. The network-pathway analysis of miRNAs and their targets was performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) bioinformatic tools to determine the potential candidate miRNAs that promote endothelial differentiation. Based on these analyses, six upregulated miRNAs (miR-181a-5p, miR-330-5p, miR-335-3p, miR-15b-5p, miR-99a-5p, and miR-199a-5p) and six downregulated miRNAs (miR-145-5p, miR-155-5p, miR-193a-3p, miR-125a-5p, miR-221-5p, and miR-222-3p) were chosen for further studies. In vitro evaluation of these miRNAs to induce endothelial differentiation when transfected into CD34+ sorted ASCs was studied using Von Willebrand Factor (VWF) staining and quantitative real time-polymerase chain reaction (qRT-PCR). Our results suggest that miRNAs: 335-5p, 330-5p, 181a-5p and anti-miRNAs: 125a-5p, 145-5p can likely induce endothelial differentiation in CD34+ sorted ASCs. Further studies are clearly required to elucidate the specific mechanisms on how miRNAs or anti-miRNAs identified through bioinformatics approach can induce the endotheliogenesis in ASCs.

Indexed as

Antigens, CD34Cell DifferentiationCells, CulturedCell SeparationEndothelial CellsFlow CytometryGene Expression ProfilingGene Regulatory NetworksHumansMesenchymal Stem CellsMicroRNAsNitric Oxide Synthase Type IIIPlatelet Endothelial Cell Adhesion Molecule-1Reverse Transcriptase Polymerase Chain ReactionRNA-SeqSignal TransductionAntigens, CD34MicroRNAsNitric Oxide Synthase Type IIIPlatelet Endothelial Cell Adhesion Molecule-1von Willebrand Factoradipose stem cellsCD34+endotheliogenesisKyoto Encyclopedia of Genes and Genomes (KEGG)miRNA-Seq

Identifiers

PMID33397204
PMCPMC7994430
OpenAlexW3118681500

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.