ArticleThe journal of physical chemistry. B2021
Discriminating between Concerted and Sequential Allosteric Mechanisms by Comparing Equilibrium and Kinetic Hill Coefficients.
Article in The journal of physical chemistry. B, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Relationship between Decimal Hill Coefficient, Intermediate Processes, and Mesoscopic Fluctuations in Gene Expression.ACS omega · 2025Article
- Cooperative & competitive binding of anti-myosin tail antibodies revealed by super-resolution microscopy.Archives of biochemistry and biophysics · 2023Article
- Mathematical models describing oxygen binding by hemoglobin.Biophysical reviews · 2023Review
- A New Model of Hemoglobin Oxygenation.Entropy (Basel, Switzerland) · 2022Article
- Partitioning the Hill coefficient into contributions from ligand-promoted conformational changes and subunit heterogeneity.Protein science : a publication of the Protein Society · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hill coefficients, which provide a measure of cooperativity in ligand binding, can be determined for equilibrium (or steady-state) data by measuring fractional saturation (or initial reaction velocities) as a function of ligand concentration. Hill coefficients can also be determined for transient kinetic data from plots of the observed rate constant of the ligand-promoted conformational change as a function of ligand concentration. Here, it is shown that the ratio of the values of these two Hill coefficients can provide insight into the allosteric mechanism. Cases when the value of the kinetic Hill coefficient is equal to or greater than the value of the equilibrium coefficient indicate concerted transitions whereas ratios smaller than one indicate a sequential transition. The derivations in this work are for symmetric dimers but are expected to have general applicability for homo-oligomers.
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