ReviewMolecular biology reports2021
Tissue culture-induced DNA methylation in crop plants: a review.
Review in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 76 citations in OpenAlex.
- Influence of the In Vitro Culture System on the Preferential Accumulation of Bioactive Compounds inPlants (Basel, Switzerland) · 2026Article
- Somatic embryogenesis-induced epigenetic changes promoting catechin accumulation in Vaccinium vitis-idaea L.Planta · 2026Article
- Cytogenomic profiling ofJournal of ginseng research · 2026Article
- Maintaining genetic stability in sweet potato: epigenetic insights into propagation and drought tolerance.Frontiers in plant science · 2026Article
- Genotype and environment synergistically determine the polyphenolic composition and functional activity of highbush blueberries.Scientific reports · 2025Article
- Molecular insights of somatic embryogenesis in coconut.Molecular biology reports · 2025Review
- A Comparison of DNA-Methylation during Protoplast Culture of Ponkan Mandarin (Plants (Basel, Switzerland) · 2024Article
- Article
- Secondary metabolite fingerprinting, anti-pathogenic activity, elite chemotype selection and conservation of3 Biotech · 2024Article
- An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants.BMC plant biology · 2024Article
- Comparative restriction enzyme analysis of methylation (CREAM) reveals methylome variability within a clonalFrontiers in plant science · 2024Article
- Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally.International journal of molecular sciences · 2023Article
- Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation.Current issues in molecular biology · 2023Article
- Light-Dependent Expression and Promoter Methylation of the Genes Encoding Succinate Dehydrogenase, Fumarase, and NAD-Malate Dehydrogenase in Maize (International journal of molecular sciences · 2023Article
- Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures.Biology · 2023Article
- Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application.Plants (Basel, Switzerland) · 2022Review
- Enhancement of healthful novel sugar contents in genetically engineered sugarcane juice integrated with molecularly characterized ThSyGII (CEMB-SIG2).Scientific reports · 2022Article
- The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Plants (Basel, Switzerland) · 2022Article
- The Epigenetic Regulation in Plant Specialized Metabolism: DNA Methylation Limits PaclitaxelFrontiers in plant science · 2022Article
- Phenotypic variation and epigenetic insight into tissue culture berry crops.Frontiers in plant science · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant tissue culture techniques have been extensively employed in commercial micropropagation to provide year-round production. Tissue culture regenerants are not always genotypically and phenotypically similar. Due to the changes in the tissue culture microenvironment, plant cells are exposed to additional stress which induces genetic and epigenetic instabilities in the regenerants. These changes lead to tissue culture-induced variations (TCIV) which are also known as somaclonal variations to categorically specify the inducing environment. TCIV includes molecular and phenotypic changes persuaded in the in vitro culture due to continuous sub-culturing and tissue culture-derived stress. Epigenetic variations such as altered DNA methylation pattern are induced due to the above-mentioned factors. Reportedly, alteration in DNA methylation pattern is much more frequent in the plant genome during the tissue culture process. DNA methylation plays an important role in gene expression and regulation of plant development. Variants originated in tissue culture process due to heritable methylation changes, can contribute to intra-species phenotypic variation. Several molecular techniques are available to detect DNA methylation at different stages of in vitro culture. Here, we review the aspects of TCIV with respect to DNA methylation and its effect on crop improvement programs. It is anticipated that a precise and comprehensive knowledge of molecular basis of in vitro-derived DNA methylation will help to design strategies to overcome the bottlenecks of micropropagation system and maintain the clonal fidelity of the regenerants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.