ArticleEuropean journal of orthopaedic surgery & traumatology : orthopedie traumatologie2021
Direct detection of microorganisms in sonicated orthopedic devices after in vitro biofilm production and different processing conditions.
Article in European journal of orthopaedic surgery & traumatology : orthopedie traumatologie, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Reducing Biofilm Infections in Burn Patients' Wounds and Biofilms on Surfaces in Hospitals, Medical Facilities and Medical Equipment to Improve Burn Care: A Systematic Review.International journal of environmental research and public health · 2021Pooled it
- Effect of sonication on staphylococcal and gram-negative biofilms relevant to prosthetic joint infections: an in vitro study.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- CApEsid biOfilm: a suggested pipeline for clinical phage microbiology for biofilm infections based on comparative method study.Scientific reports · 2026Article
- Evaluating the efficacy of delafloxacin and combination therapy against Staphylococcus aureus Biofilms: insights into activity in acidic environments.Scientific reports · 2026Article
- A Novel Approach for Tissue Analysis in Joint Infections Using the Scattered Light Integrating Collector (SLIC).Biosensors · 2025Article
- Additive Manufacturing, Thermoplastics, CAD Technology, and Reverse Engineering in Orthopedics and Neurosurgery-Applications to Preventions and Treatment of Infections.Antibiotics (Basel, Switzerland) · 2025Review
- Validation of Lyophilized Human Fecal Microbiota for the Treatment ofMicroorganisms · 2024Article
- eHealth and mHealth in Antimicrobial Stewardship to Reduce Mortality in Empirical Antimicrobial Therapy and a Systematic Review with a Meta-Analysis of Adequate Therapy.Infectious disease reports · 2024Review
- Sonication protocols and their contributions to the microbiological diagnosis of implant-associated infections: a review of the current scenario.Frontiers in cellular and infection microbiology · 2024Review
- Evaluation of Staphylococcus aureus and Candida albicans biofilms adherence to PEEK and titanium-alloy prosthetic spine devices.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2022Article
- eHealth and mHealth in Antimicrobial Stewardship Programs.Digital biomarkersReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe gold standard for microbial detection in prosthetic joint infections is the multiple culture of the peri-prosthetic tissue. The fluid cultures after sonication can improve the recovery of the microorganisms.
objectiveThe aim of this study was to evaluate the sonication technique with a plastic bag and the effect of refrigeration on microorganism detection with conventional culturing, MALDI-TOF MS and qPCR assay on an orthopedic screw model.
methodsWe produced biofilms of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans on orthopedic screws, which were stored under different conditions and temperatures before sonication. After sonication, the mass spectrometry by MALDI-TOF, qPCR and culture protocols was performed using the sonicated fluid, for detecting the microorganisms involved in the biofilm.
resultsThe bacterial bioburden decreased by approximately one log after the refrigeration period, in the screws containing P. aeruginosa and S. aureus biofilms. All the microorganisms involved in the screw biofilms were detected with MALDI-TOF and qPCR. Significant reductions in CFU counts occurred only in groups stored in the plastic bag, indicating that changes in temperature and humidity may favor cell death. However, this variation is not important for this model as it did not affect the detection owing to the high counts obtained.
conclusionMicrobial identification by MALDI-TOF in sonicated fluid is feasible. With qPCR, there were no differences between the detection in the screws processed immediately or after refrigeration. It is necessary to consider whether or not the refrigeration period would affect microbial recovery in an explanted prosthesis.
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