Evidence map›Paper›PMID 33392922›Full record

ArticleMolecular and cellular biochemistry2021

Elucidation of underlying molecular mechanism of 5-Fluorouracil chemoresistance and its restoration using fish oil in experimental colon carcinoma.

Isha Rani, Sandeep Kumar, Bhoomika Sharma, Rajendra Prasad, Satinder Kaur, Prerna Sharma, Navneet Agnihotri

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Article in Molecular and cellular biochemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Membrane Stress and Ferroptosis: Lipid Dynamics in Cancer.International journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Isha RaniDepartment of Biochemistry, M.M. Institute of Medical Sciences and Research (MMIMSR), Mullana, Ambala, Haryana, India.
Sandeep KumarDepartment of Biochemistry, Panjab University, Chandigarh, 160025, India.
Bhoomika SharmaDepartment of Biochemistry, Panjab University, Chandigarh, 160025, India.
Rajendra PrasadDepartment of Biochemistry, M.M. Institute of Medical Sciences and Research (MMIMSR), Mullana, Ambala, Haryana, India.
Satinder KaurDepartment of Biochemistry, Panjab University, Chandigarh, 160025, India.
Prerna SharmaDepartment of Biochemistry, Panjab University, Chandigarh, 160025, India.
Navneet AgnihotriDepartment of Biochemistry, Panjab University, Chandigarh, 160025, India. agnihotri.navneet@gmail.com.ORCID http://orcid.org/0000-0001-6063-3473
Panjab University · INInstitute of Medical Sciences · IN

Funding

DST, India SR/SO/BB-0138/2012
6 · The paper itself

Abstract

Latest strategies for cancer treatment primarily focus on the use of chemosensitizers to enhance therapeutic outcome. N-3 PUFAs have emerged as the strongest candidate for the prevention of colorectal cancer (CRC). Our previous studies have demonstrated that fish oil (FO) rich in n-3 PUFAs not only increased therapeutic potential of 5-Fluorouracil(5-FU) in colon cancer but also ameliorated its toxicity. Henceforth, the present study is designed to elucidate mechanistic insights of FO as a chemosensitizer to circumvent drug resistance in experimental colon carcinoma. The colon cancer was induced by 1,2-dimethylhydrazine(DMH)/dextran sulfate sodium(DSS) in male Balb/c mice and these animals were treated with 5-FU(12.5 mg/kg b.w.), FO(0.2 ml), or 5-FU + FO(12.5 mg/kg b.w + 0.2 ml) orally for 14 days. The molecular mechanism of overcoming 5-FU resistance using FO in colon cancer was delineated by estimating expression of cancer stem cell markers using flowcytometric method and drug transporters by immunohistochemistry and immunoblotting. Additionally, distribution profile of 5-FU and its cytotoxic metabolite, 5-FdUMP at target(colon), and non-target sites (serum, kidney, liver, spleen) was assessed using high-performance liquid chromatography(HPLC) method. The observations revealed that expression of CSCs markers was remarkably reduced after using fish oil along with 5-FU in carcinogen-treated animals. Interestingly, the use of FO alongwith 5-FU also significantly declined the expression of drug transporters (ABCB1,ABCC5) and consequently resulted in an increased cellular uptake of 5-FU and its metabolite, 5-FdUMP at target site (colon). It could be possibly associated with change in permeability of cell membrane owing to the alteration in membrane fluidity. The present study revealed the mechanistic insights of FO as a MDR revertant which successfully restored 5-FU-mediated chemoresistance in experimental colon carcinoma.

Indexed as

Drug Resistance, Neoplasm1,2-DimethylhydrazineAnimalsAntimetabolites, AntineoplasticAntineoplastic AgentsATP-Binding Cassette, Sub-Family C ProteinsATP Binding Cassette Transporter, Subfamily B, Member 1Cell MembraneColonColonic NeoplasmsDextran SulfateFatty Acids, Omega-3Fish OilsFluorouracilHumansMale1,2-DimethylhydrazineAbcc5 protein, mouseAntimetabolites, AntineoplasticAntineoplastic AgentsATP-Binding Cassette, Sub-Family C ProteinsATP Binding Cassette Transporter, Subfamily B, Member 1Dextran SulfateFatty Acids, Omega-3Fish OilsFluorouracil5-Fluoro-2′-deoxyuridine 5′ monophosphate (5-FdUMP)5-Fluorouracil (5-FU)ATP-binding cassette (ABC) transportersColorectal cancer (CRC)Fish oil (FO)Multi drug resistance (MDR)

Identifiers

PMID33392922
OpenAlexW3118792867

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.