ArticlePlanta2021
Comparative RNA-Seq analysis reveals genes associated with masculinization in female Cannabis sativa.
Article in Planta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 48 citations in OpenAlex.
- Anatomical and Ontogenetic Basis of Silver Thiosulfate-Induced Masculinization in Genetically FemalePlants (Basel, Switzerland) · 2026Article
- Genetic Advances inPlants (Basel, Switzerland) · 2026Review
- Transcriptomic dissection of floral identity and trichome-derived metabolite pathways in Cannabis sativa L.Planta · 2026Article
- Hermaphroditism inPlants (Basel, Switzerland) · 2026Review
- Weeding out variability: a proof-of-concept for producing uniform FHorticulture research · 2026Article
- Harnessing Sex Reversion via Chemical Intervention inPlants (Basel, Switzerland) · 2026Article
- Network ontology transcript annotation identifies genetic signals underlying sex determination.Scientific reports · 2026Article
- Transcriptomic insights into unisexual flower development in Cannabis sativa: conserved MADS-box networks and divergent developmental programs.BMC plant biology · 2026Article
- Article
- Sex-specific ethylene responses drive floral sexual plasticity in Cannabis sativa.The Plant journal : for cell and molecular biology · 2026Article
- Identification of circRNAs in industrial hemp (Frontiers in plant science · 2026Article
- Gibberellin pathway remodeling accompanies ethylene-driven sex reversal inFrontiers in plant science · 2026Article
- Integrative analysis of transcriptome and metabolome profiles reveals immune-metabolic alterations in pulmonary sarcoidosis.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Dynamic gene regulatory networks drive seed dormancy and germination of Pinus tabuliformis.BMC plant biology · 2025Article
- From fibers to flowering to metabolites: unlocking hemp (Cannabis sativa) potential with the guidance of novel discoveries and tools.Journal of experimental botany · 2025Review
- Identification of genes associated with sex expression and sex determination in hemp (Cannabis sativa L.).Journal of experimental botany · 2025Article
- Article
- Reactive Oxygen and Related Regulatory Factors Involved in Ethylene-Induced Petal Abscission in Roses.Plants (Basel, Switzerland) · 2024Article
- Cosexuality Reduces Pollen Production and Fitness inPlants (Basel, Switzerland) · 2023Article
- Highly sex specific gene expression in Jojoba.BMC plant biology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionUsing RNA profiling, we identified several silver thiosulfate-induced genes that potentially control the masculinization of female Cannabis sativa plants. Genetically female Cannabis sativa plants normally bear female flowers, but can develop male flowers in response to environmental and developmental cues. In an attempt to elucidate the molecular elements responsible for sex expression in C. sativa plants, we developed genetically female lines producing both female and chemically-induced male flowers. Furthermore, we carried out RNA-Seq assays aimed at identifying differentially expressed genes responsible for male flower development in female plants. The results revealed over 10,500 differentially expressed genes, of which around 200 potentially control masculinization of female cannabis plants. These genes include transcription factors and other genes involved in male organ (i.e., anther and pollen) development, as well as genes involved in phytohormone signalling and male-biased phenotypes. The expressions of 15 of these genes were further validated by qPCR assay confirming similar expression patterns to that of RNA-Seq data. These genes would be useful for understanding predisposed plants producing flowers of both sex types in the same plant, and help breeders to regulate the masculinization of female plants through targeted breeding and plant biotechnology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.