ArticleFrontiers in plant science2020
High-Pressure-Sprayed Double Stranded RNA Does Not Induce RNA Interference of a Reporter Gene.
Article in Frontiers in plant science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- The unfulfilled potential of nanocarriers for RNA delivery in antiviral crop protection.Nature plants · 2026Review
- Control Strategies of Plant Viruses Using Spray-Induced Gene Silencing.The plant pathology journal · 2026Review
- Exogenous 24nt siRNAs induce AGO4A-dependent silencing via promoter DNA methylation and H3K9me2 deposition.Frontiers in plant science · 2026Article
- Regulation of Plant Genes with Exogenous RNAs.International journal of molecular sciences · 2025Review
- Cross-kingdom RNAi induced by a beneficial endophytic fungus to its host requires transitivity and amplification of silencing signals.Plant biology (Stuttgart, Germany) · 2025Article
- Agrochemical innovation for crop health: moving forward through dynamic disorder.Frontiers in plant science · 2025Article
- Exploring the challenges of RNAi-based strategies for crop protection.Advanced biotechnology · 2024Review
- Engineered Resistance to Tobamoviruses.Viruses · 2024Review
- Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea.Molecular plant pathology · 2024Review
- Leveraging RNA interference technology for selective and sustainable crop protection.Frontiers in plant science · 2024Review
- Characterisation and functionalisation of chitosan nanoparticles as carriers for double-stranded RNA (dsRNA) molecules towards sustainable crop protection.Bioscience reports · 2023Article
- A Non-Canonical Pathway Induced by Externally Applied Virus-Specific dsRNA in Potato Plants.International journal of molecular sciences · 2023Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Recent Progress on Nanocarriers for Topical-Mediated RNAi Strategies for Crop Protection-A Review.Molecules (Basel, Switzerland) · 2023Review
- Small RNA-seq dataset of wild type and 16CData in brief · 2022Article
- RNAi as a Foliar Spray: Efficiency and Challenges to Field Applications.International journal of molecular sciences · 2022Review
- Exogenous dsRNA Induces RNA Interference of a Chalcone Synthase Gene inInternational journal of molecular sciences · 2022Article
- Small RNA-based plant protection against diseases.Frontiers in plant science · 2022Review
- External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation inInternational journal of molecular sciences · 2021Article
- Towards responsible communication of agricultural biotechnology research for the common good.Nature reviews. Molecular cell biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, RNA interference (RNAi) is an effective defense mechanism against pathogens and pests. RNAi mainly involves the micro RNA and the small interfering RNA (siRNA) pathways. The latter pathway is generally based on the processing of long double stranded RNAs (dsRNA) into siRNAs by DICER-LIKE endonucleases (DCLs). SiRNAs are loaded onto ARGONAUTE proteins to constitute the RNA-induced silencing complex (RISC). Natural dsRNAs derive from transcription of inverted repeats or of specific RNA molecules that are transcribed by RNA-directed RNA polymerase 6 (RDR6). Moreover, replication of infecting viruses/viroids results in the production of dsRNA intermediates that can serve as substrates for DCLs. The high effectiveness of RNAi both locally and systemically implicated that plants could become resistant to pathogens, including viruses, through artificial activation of RNAi by topical exogenous application of dsRNA. The most preferable procedure to exploit RNAi would be to simply spray naked dsRNAs onto mature plants that are specific for the attacking pathogens serving as a substitute for pesticides applications. However, the plant cell wall is a difficult barrier to overcome and only few reports claim that topical application of naked dsRNA triggers RNAi in plants. Using a transgenic
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.