ArticleFrontiers in microbiology2020
Fast and Easy Phage-Tagging and Live/Dead Analysis for the Rapid Monitoring of Bacteriophage Infection.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Stable co-existence ofmBio · 2026Article
- The opposite effects of stringent response on phage infection ofmicroLife · 2026Article
- Phage P100 resistance in clinical and foodborneCurrent research in microbial sciences · 2026Article
- Engineered bacteriophage-based bioimaging Technology: Development and applications.Synthetic and systems biotechnology · 2025Review
- Probiotic Antimicrobial Evaluation Via Real-Time Profiling of Bacterial Cell Proliferation Using Stochastic Kinetics.ACS sensors · 2025Article
- Susceptibility profile of clinical and food-associatedFrontiers in microbiology · 2025Article
- ColorPhAST: a visual rapid colorimetric assay for detecting phage-susceptibility inFrontiers in microbiology · 2025Article
- Microscopic phage adsorption assay: High-throughput quantification of virus particle attachment to host bacterial cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Evolution of pH-sensitive transcription termination inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- An electrochemical biosensor for sensitive detection of live Salmonella in food via MXene amplified methylene blue signals and electrostatic immobilization of bacteriophages.Mikrochimica acta · 2024Article
- Serratia marcescens ATCC 274 increases production of the red pigment prodigiosin in response to Chi phage infection.Scientific reports · 2024Article
- Polymeric nanocarrier-based adjuvants to enhance a locally produced mucosal coryza vaccine in chicken.Scientific reports · 2024Article
- Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry.Frontiers in microbiology · 2024Article
- A comparison of phage susceptibility testing with two liquid high-throughput methods.Frontiers in microbiology · 2024Article
- Monitoring phage-induced lysis of gram-negatives in real time using a fluorescent DNA dye.Scientific reports · 2023Article
- Phage-Host Interaction Analysis by Flow Cytometry Allows for Rapid and Efficient Screening of Phages.Antibiotics (Basel, Switzerland) · 2022Article
- Rapid hydrogel-based phage susceptibility test for pathogenic bacteria.Frontiers in cellular and infection microbiology · 2022Article
- Determination of phage susceptibility as a clinical diagnostic tool: A routine perspective.Frontiers in cellular and infection microbiology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Use of bacteriophages, which are viruses that kill bacteria, for biocontrol of pathogens and antimicrobial resistant bacteria has become increasingly important in recent years. As traditional culture-based methods are laborious and time-consuming, practicable use of bacteriophages will hinge on development of rapid and high throughput methods to analyze, characterize and screen large bacteriophage libraries. We thus established a novel method to fluorescently tag bacteriophages for virus screening and interaction studies, without the need for complicated and laborious purification procedures or genetic engineering of viruses to express fluorescent proteins. Bacteriophage PMBT14 was tagged using DNA dye Syto 13. Simply by using a membrane filter, tagged bacteriophages can be separated from non-sequestered excess dye rapidly, effortlessly, and cheaply. The procedure takes less than 30 min and makes use of simple laboratory consumables that are already commonly used for bacteriophage preparations. As proof of concept, we present here flow cytometric methods to analyze bacteriophage binding, infection and killing that are very accessible for high throughput analysis. We show that the resulting fluorescently tagged bacteriophage can be used to specifically stain its host bacterium
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.