ArticleThe Journal of cell biology1988
The polarity and stability of microtubule capture by the kinetochore.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Kinetochores get a grip!The Journal of cell biology · 2025Article
- Kinetochores grip microtubules with directionally asymmetric strength.The Journal of cell biology · 2025Article
- Article
- Self-organized optical device driven by motor proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- A role for regulated binding of p150(Glued) to microtubule plus ends in organelle transport.The Journal of cell biology · 2002Article
- The dynamic behavior of individual microtubules associated with chromosomes in vitro.Molecular biology of the cell · 1998Article
- Kinetochore microtubule dynamics and the metaphase-anaphase transition.The Journal of cell biology · 1995Article
- Making first contacts between the spindle and the chromosomes in HeLa cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1994Article
- Microinjection of biotin-tubulin into anaphase cells induces transient elongation of kinetochore microtubules and reversal of chromosome-to-pole motion.The Journal of cell biology · 1992Article
- A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Modulation of microtubule stability by kinetochores in vitro.The Journal of cell biology · 1990Article
- Article
- Ciliary microtubule capping structures contain a mammalian kinetochore antigen.The Journal of cell biology · 1990Article
- Identification of a kinesin-like microtubule-based motor protein in Dictyostelium discoideum.The EMBO journal · 1989Article
- Biotin-tubulin incorporates into kinetochore fiber microtubules during early but not late anaphase.The Journal of cell biology · 1989Article
- Microtubule polarities indicate that nucleation and capture of microtubules occurs at cell surfaces in Drosophila.The Journal of cell biology · 1989Article
- Toxoplasma gondii actively remodels the microtubule network in host cells.Microbes and infectionArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the capture of microtubules by isolated metaphase chromosomes, using microtubules stabilized with taxol and marked with biotin tubulin to distinguish their plus and minus ends. The capture reaction is reversible at both the plus and minus ends. The on rate of capture is the same for both polarities but the dissociation rate from the kinetochore is seven times slower with microtubules captured at their plus ends than those captured at their minus ends. At steady state this disparity in off rates leads to the gradual replacement of microtubules captured at their minus ends with those captured at their plus ends. These results suggest that the kinetochore makes a lateral attachment near the end of the microtubule in the initial capture reaction and shows a structural specificity that may be important in proper bipolar attachment of the chromosome to the spindle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.