ArticleAnalytical and bioanalytical chemistry2021
Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Visualizing the molecular landscape of skin: applications and perspectives of mass spectrometry imaging.Analytical and bioanalytical chemistry · 2026Review
- Review
- Lipidomic signatures of CNS ischemic injury and their modulation by immunomodulatory hydrogels.The Analyst · 2026Article
- Optical, Tomographic, and Mass Spectrometry Imaging Methods for Burn Wounds: Capabilities, Limitations, and Clinical Potential.Biomedicines · 2026Review
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Unraveling the molecular dynamics of wound healing: integrating spatially resolved lipidomics and temporally resolved proteomics.Analytical and bioanalytical chemistry · 2025Article
- Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction.Analytical and bioanalytical chemistry · 2025Article
- Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain.Analytical and bioanalytical chemistry · 2024Article
- Mass Spectrometry Imaging of Cholesterol and Oxysterols.Advances in experimental medicine and biology · 2024Article
- Time of acquisition and high spatial resolution mass spectrometry imaging.Journal of mass spectrometry : JMS · 2023Article
- Transforming a Mid-infrared Laser Profile from Gaussian to a Top-Hat with a Diffractive Optical Element for Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2023Article
- Quasi-continuous infrared matrix-assisted laser desorption electrospray ionization source coupled to a quadrupole time-of-flight mass spectrometer for direct analysis from well plates.Journal of mass spectrometry : JMS · 2023Article
- The development and application of matrix assisted laser desorption electrospray ionization: The teenage years.Mass spectrometry reviews · 2023Review
- Improved spatial resolution of infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective.Rapid communications in mass spectrometry : RCM · 2022Article
- Article
- Next-Generation Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Source for Mass Spectrometry Imaging and High-Throughput Screening.Journal of the American Society for Mass Spectrometry · 2022Article
- Novel matrix strategies for improved ionization and spatial resolution using IR-MALDESI mass spectrometry imaging.Journal of mass spectrometry : JMS · 2022Article
- Multimodal Mass Spectrometry Imaging of Rat Brain Using IR-MALDESI and NanoPOTS-LC-MS/MS.Journal of proteome research · 2022Article
- Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization.Metabolites · 2021Article
- Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis.Metabolites · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Three-dimensional (3D) mass spectrometry imaging (MSI) has become a growing frontier as it has the potential to provide a 3D representation of analytes in a label-free, untargeted, and chemically specific manner. The most common 3D MSI is accomplished by the reconstruction of 2D MSI from serial cryosections; however, this presents significant challenges in image alignment and registration. An alternative method would be to sequentially image a sample by consecutive ablation events to create a 3D image. In this study, we describe the use of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in ablation-based 3D MSI for analyses of lipids within fresh frozen skin tissue. Depth resolution using different laser energy levels was explored with a confocal laser scanning microscope to establish the imaging parameters for skin. The lowest and highest laser energy level resulted in a depth resolution of 7 μm and 18 μm, respectively. A total of 594 lipids were putatively detected and detailed lipid profiles across different skin layers were revealed in a 56-layer 3D imaging experiment. Correlated with histological information, the skin structure was characterized with differential lipid distributions with a lateral resolution of 50 μm and a z resolution of 7 μm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.