ArticleBrain structure & function2021
Optimization and evaluation of fluorescence in situ hybridization chain reaction in cleared fresh-frozen brain tissues.
Article in Brain structure & function, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- Enhanced Precision of Fluorescence In Situ Hybridization (FISH) Analysis Using Neural Network-Based Nuclear Segmentation for Digital Microscopy Samples.Sensors (Basel, Switzerland) · 2026Article
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- Whole-brain mapping in adult zebrafish and identification of a novel tank test functional connectome.bioRxiv : the preprint server for biology · 2024Article
- An Improved Nested U-Net Network for Fluorescence In Situ Hybridization Cell Image Segmentation.Sensors (Basel, Switzerland) · 2024Article
- Whole-brain mapping reveals the divergent impact of ketamine on the dopamine system.Cell reports · 2023Article
- Challenges and opportunities to computationally deconvolve heterogeneous tissue with varying cell sizes using single-cell RNA-sequencing datasets.Genome biology · 2023Review
- High emotional reactivity is associated with activation of a molecularly distinct hippocampal-amygdala circuit modulated by the glucocorticoid receptor.Neurobiology of stress · 2023Article
- Article
- Effect of Selective Lesions of Nucleus Accumbens µ-Opioid Receptor-Expressing Cells on Heroin Self-Administration in Male and Female Rats: A Study with NovelThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Optical Tissue Clearing: Illuminating Brain Function and Dysfunction.Theranostics · 2021Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Transcript labeling in intact tissues using in situ hybridization chain reaction has potential to provide vital spatiotemporal information for molecular characterization of heterogeneous neuronal populations. However, large tissue labeling in non-perfused or fresh-frozen rodent and postmortem human samples, which provide more flexible utilization than perfused tissues, is largely unexplored. In the present study, we optimized the combination of in situ hybridization chain reaction in fresh-frozen rodent brains and then evaluated the uniformity of neuronal labeling between two clearing methods, CLARITY and iDISCO
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Registered trials
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