ArticleGenomics, proteomics & bioinformatics2020
Pooled Plasmid Sequencing Reveals the Relationship Between Mobile Genetic Elements and Antimicrobial Resistance Genes in Clinically Isolated Klebsiella pneumoniae.
Article in Genomics, proteomics & bioinformatics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Genomic insights into multidrug-resistant bacteria: Emergence ofVirulence · 2026Article
- Mobile Genetic Elements as Central Drivers of Antimicrobial Resistance: Molecular Mechanisms, Evolutionary Ecology, One Health Implications and Control Strategies.Antibiotics (Basel, Switzerland) · 2026Review
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- Cross-species transmission ofFrontiers in cellular and infection microbiology · 2026Review
- Large-scale plasmidome and carbapenem mobilome analysis reveals a mechanistic duality: high-power specialists and structural generalists mobile genetic elements.Frontiers in microbiology · 2026Article
- The emergence of chromosomally locatedFrontiers in microbiology · 2026Article
- Shifts inMicrobiology spectrum · 2025Article
- Diversity of antibiotic resistance genes and mobile genetic elements of Sof Umer Cave microbiomes, Ethiopia.BMC genomic data · 2025Article
- Mobile genetic elements inJournal of bacteriology · 2025Review
- Global diversity and distribution of antibiotic resistance genes in human wastewater treatment systems.Nature communications · 2025Article
- Genomic analyses reveal presence of extensively drug-resistantFrontiers in microbiology · 2025Article
- Antibiotic resistance in livestock, environment and humans: One Health perspective.Journal of animal science and technology · 2024Review
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- Whole Genome Sequencing and Molecular Analysis of Carbapenemase-ProducingMicroorganisms · 2022Article
- Complete Genome Sequence of Klebsiella quasipneumoniae MMCC7, Isolated from an Eclectus Parrot (Eclectus roratus).Microbiology resource announcements · 2022Article
- Genome-Wide Association Study Reveals Host Factors Affecting Conjugation inMicroorganisms · 2022Article
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- Genetic Diversity and Characteristics ofFrontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmids remain important microbial components mediating the horizontal gene transfer (HGT) and dissemination of antimicrobial resistance. To systematically explore the relationship between mobile genetic elements (MGEs) and antimicrobial resistance genes (ARGs), a novel strategy using single-molecule real-time (SMRT) sequencing was developed. This approach was applied to pooled conjugative plasmids from clinically isolated multidrug-resistant (MDR) Klebsiella pneumoniae from a tertiary referral hospital over a 9-month period. The conjugative plasmid pool was obtained from transconjugants that acquired antimicrobial resistance after plasmid conjugation with 53 clinical isolates. The plasmid pool was then subjected to SMRT sequencing, and 82 assembled plasmid fragments were obtained. In total, 124 ARGs (responsible for resistance to β-lactam, fluoroquinolone, and aminoglycoside, among others) and 317 MGEs [including transposons (Tns), insertion sequences (ISs), and integrons] were derived from these fragments. Most of these ARGs were linked to MGEs, allowing for the establishment of a relationship network between MGEs and/or ARGs that can be used to describe the dissemination of resistance by mobile elements. Key elements involved in resistance transposition were identified, including IS26, Tn3, IS903B, ISEcp1, and ISKpn19. As the most predominant IS in the network, a typical IS26-mediated multicopy composite transposition event was illustrated by tracing its flanking 8-bp target site duplications (TSDs). The landscape of the pooled plasmid sequences highlights the diversity and complexity of the relationship between MGEs and ARGs, underpinning the clinical value of dominant HGT profiles.
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