ArticleMedical & biological engineering & computing2021
Quantitative analysis of blood cells from microscopic images using convolutional neural network.
Article in Medical & biological engineering & computing, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed, 17 citations in OpenAlex.
- Convolutional neural network-based automatic classification for incomplete antibody reaction intensity in solid phase anti-human globulin test image.Medical & biological engineering & computing · 2022Article
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4 authors at 1 institution in 3 countries.
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Abstract
Blood cell count provides relevant clinical information about different kinds of disorders. Any deviation in the number of blood cells implies the presence of infection, inflammation, edema, bleeding, and other blood-related issues. Current microscopic methods used for blood cell counting are very tedious and are highly prone to different sources of errors. Besides, these techniques do not provide full information related to blood cells like shape and size, which play important roles in the clinical investigation of serious blood-related diseases. In this paper, deep learning-based automatic classification and quantitative analysis of blood cells are proposed using the YOLOv2 model. The model was trained on 1560 images and 2703-labeled blood cells with different hyper-parameters. It was tested on 26 images containing 1454 red blood cells, 159 platelets, 3 basophils, 12 eosinophils, 24 lymphocytes, 13 monocytes, and 28 neutrophils. The network achieved detection and segmentation of blood cells with an average accuracy of 80.6% and a precision of 88.4%. Quantitative analysis of cells was done following classification, and mean accuracy of 92.96%, 91.96%, 88.736%, and 92.7% has been achieved in the measurement of area, aspect ratio, diameter, and counting of cells respectively.Graphical abstract Graphical abstract where the first picture shows the input image of blood cells seen under a compound light microscope. The second image shows the tools used like OpenCV to pre-process the image. The third image shows the convolutional neural network used to train and perform object detection. The 4th image shows the output of the network in the detection of blood cells. The last images indicate post-processing applied on the output image such as counting of each blood cells using the class label of each detection and quantification of morphological parameters like area, aspect ratio, and diameter of blood cells so that the final result provides the number of each blood cell types (seven) and morphological information providing valuable clinical information.
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