ArticleiScience2021
The core circadian component, Bmal1, is maintained in the pineal gland of old killifish brain.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Unlocking the potential of circadian biology for cardiovascular health.Physiological reviews · 2026Review
- Zebrafish as a translational filter for natural sleep modulators.Frontiers in nutrition · 2026Review
- Aging Reduces Intestinal Stem Cell Activity in Killifish and Intermittent Fasting Reverses Intestinal Gene Expression Patterns.Aging cell · 2025Article
- Organization of Serotonergic Cell Populations in the Brain and Spinal Cord of the Short-Lived African Turquoise Killifish.Biology · 2025Article
- Age-associated spinal stenosis in the turquoise killifish.iScience · 2023Article
- Melanopsin-mediated optical entrainment regulates circadian rhythms in vertebrates.Communications biology · 2023Review
- Review
- Importance of Bmal1 in Alzheimer's disease and associated aging-related diseases: Mechanisms and interventions.Aging cell · 2022Review
- Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing.STAR protocols · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circadian rhythm is altered during aging, although the underlying molecular mechanisms remain largely unknown. Here, we used the turquoise killifish as a short-lived vertebrate model to examine the effects of aging on the major circadian network comprising the four mammalian clock protein homologs, Bmal1, Clockb, Cry1b, and Per3, which are highly conserved in the killifish with 50%-85% amino acid sequence identity to their human counterparts. The amplitude of circadian rhythm was smaller in old fish (14 weeks) than in young fish (6 weeks). In old fish brain, the Bmal1 protein level was significantly downregulated. However, the Bmal1 interaction with Clockb and chromatin binding of Bmal1 to its downstream target promoters were retained. Furthermore, Bmal1 was relatively well maintained in the pineal gland compared with other regions of the old fish brain. The results suggest that the circadian clock system in the killifish becomes spatially confined to the pineal gland upon aging.
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