ArticleTranslational vision science & technology2020
Alginate- and Hyaluronic Acid-Based Hydrogels as Vitreous Substitutes: An In Vitro Evaluation.
Article in Translational vision science & technology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Vitrectomy Status Alters Early Intravitreal Drug Distribution: An Ex Vivo PET-CT Study.Investigative ophthalmology & visual science · 2026Article
- Vitreous Substitutes in Vitreoretinal Surgery: From Native Vitreous Physiology to Bioengineered Experimental Replacements.Journal of functional biomaterials · 2026Review
- [Vitreous body substitutes: current solutions and future perspectives].Die Ophthalmologie · 2026Review
- Cellular Uptake of Nanoparticles is Regulated by Integrin-Based Adhesion to the Extracellular Matrix.ACS applied materials & interfaces · 2026Article
- Bridging Preclinical and Clinical Gaps in Ocular Therapeutics: Hydrogel Drug Delivery and 3D Tissue Models.International journal of nanomedicine · 2026Review
- Replacing the vitreous body with hydrogels: Rationale and strategies.Progress in retinal and eye research · 2025Review
- A Microscale-Optical Interface to Examine Electric Field-Induced Cell Motility Within Whole-Eye Facsimiles.Micro · 2025Article
- In Vitro Physicochemical and Pharmacokinetic Properties of Bevacizumab Dissolved in Silicone Oils Compared to Hydrogel-Substitutes and Porcine Vitreous Bodies.Gels (Basel, Switzerland) · 2024Article
- Viscoelastic, Optical, and Surgical Properties of Vitreous Body Replacement Hydrogels After Aging Compared to Porcine Vitreous Bodies And Silicone Oils.Translational vision science & technology · 2024Article
- Hydrogel for light delivery in biomedical applications.Bioactive materials · 2024Review
- Biopolymeric Innovations in Ophthalmic Surgery: Enhancing Devices and Drug Delivery Systems.Polymers · 2024Review
- Forward Light Scattering of First to Third Generation Vitreous Body Replacement Hydrogels after Surgical Application Compared to Conventional Silicone Oils and Vitreous Body.Gels (Basel, Switzerland) · 2023Article
- Next-generation nanomaterials: advancing ocular anti-inflammatory drug therapy.Journal of nanobiotechnology · 2023Review
- Vitreous Substitutes from Bench to the Operating Room in a Translational Approach: Review and Future Endeavors in Vitreoretinal Surgery.International journal of molecular sciences · 2023Review
- Hyaluronan Modulates the Biomechanical Properties of the Cornea.Investigative ophthalmology & visual science · 2022Article
- High-Throughput Dispensing of Viscous Solutions for Biomedical Applications.Micromachines · 2022Article
- Vitreous Substitutes as Drug Release Systems.Translational vision science & technology · 2022Review
- Hyaluronic Acid: Its Versatile Use in Ocular Drug Delivery with a Specific Focus on Hyaluronic Acid-Based Polyelectrolyte Complexes.Pharmaceutics · 2022Review
- Intravitreal Application: Physicochemical Properties of Drugs Dissolved in Silicone Oils of Different Density in Comparison to the Porcine Vitreous Body.Pharmaceutics · 2022Article
- Silicone Oil Tamponade-Retina Contact in Highly Myopic Eyes With and Without Encircling Bands: A Computational Fluid Dynamics Study.Translational vision science & technology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: To study alginate- and hyaluronic acid-based hydrogels in vitro as vitreous substitutes. Methods: Biopolymeric hydrogels based on high-molecular alginate (0.5% and 1.0%) and hyaluronic acid (1.0% and Healaflow) were compared with extracted human vitreous bodies and silicone oil (SIL-5000) regarding their optical properties (refractive index, transmission) and viscoelastic characteristics (storage modulus G', loss modulus G″). The cytotoxic (metabolic activity, apoptosis) and antiproliferative profiles were determined using cultured human fibroblasts, ARPE-19, and photoreceptor cells. The hydrogel systems were applied to human fetal retinal pigment epithelial cells cultured for two months until maximum transepithelial electrical resistance (TEER) to investigate the effect of the gel matrices on tight junctions using TEER measurements and immunostainings against the tight junction protein ZO-1. Results: Tested alginate- and hyaluronic acid-based hydrogels resembled the natural refractive index of human vitreous bodies (1.3356-1.3360) in contrast to SIL-5000 (1.4034) and showed high optical transparency (>90%) within the visible light region. The biopolymeric hydrogels exhibited viscoelastic properties similar to juvenile vitreous bodies with G'>G″ adjustable via different gelation times, contrary to SIL-5000 (G'<G″). The metabolic activity, apoptosis and tight junctions of all tested ocular cells were unaffected by the alginate- and hyaluronic acid-based vitreous substitutes. Conclusions: The present in vitro study demonstrates good optical, viscoelastic, and biocompatible properties of alginate- and hyaluronic acid-based hydrogels required for their use as vitreous substitutes. Translational Relevance: Biopolymer-based hydrogels represent a promising vitreous replacement strategy to treat vitreoretinal diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.