ArticleScientific reports2020
Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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23 citing papers in PubMed, 101 citations in OpenAlex.
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- Microbially induced calcite precipitation (MICP): mechanisms, engineering applications, and pathway to field-scale deployment - a comprehensive review.Frontiers in microbiology · 2026Review
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- Industrial-grade nitrogen sources modulate CaCO3 polymorphs and strength in MICP-cemented sand: A structure-property study.PloS one · 2026Article
- Modeling the Effect of Microbially Induced Calcium Carbonate Precipitation (MICP) on COEnvironmental science & technology · 2025Article
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- Strategies for cost-optimized biocement production: a comprehensive review.World journal of microbiology & biotechnology · 2025Review
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- Experimental study on the homogeneity of microbial grouting to reinforce calcareous sand.PloS one · 2025Article
- Bhargavaea beijingensis a promising tool for bio-cementation, soil improvement, and mercury removal.Scientific reports · 2024Article
- Single-Particle Crushing Test of Coated Calcareous Sand Based on MICP.Materials (Basel, Switzerland) · 2024Article
- Phenotypic Characterization and Draft Genome Sequence Analyses of Two Novel Endospore-FormingMicroorganisms · 2023Article
- Soil microbial improvement using enriched vinasse as a new abundant waste.Scientific reports · 2023Article
- Microbially Induced Calcium Carbonate Precipitation byApplied and environmental microbiology · 2023Review
- Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates.BMC biotechnology · 2023Article
- Biologically-induced synthetic manganese carbonate precipitate (BISMCP) for potential applications in heavy metal removal.Heliyon · 2023Article
- Assessing the capabilities of 2D fluorescence monitoring in microtiter plates with data-driven modeling for secondary substrate limitation experiments of Hansenula polymorpha.Journal of biological engineering · 2023Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial induced calcite precipitation (MICP) based on ureolysis has a high potential for many applications, e.g. restoration of construction materials. The gram-positive bacterium Sporosarcina pasteurii is the most commonly used microorganism for MICP due to its high ureolytic activity. However, Sporosarcina pasteurii is so far cultivated almost exclusively in complex media, which only results in moderate biomass concentrations at the best. Cultivation of Sporosarcina pasteurii must be strongly improved in order to make technological application of MICP economically feasible. The growth of Sporosarcina pasteurii DSM 33 was boosted by detecting auxotrophic deficiencies (L-methionine, L-cysteine, thiamine, nicotinic acid), nutritional requirements (phosphate, trace elements) and useful carbon sources (glucose, maltose, lactose, fructose, sucrose, acetate, L-proline, L-alanine). These were determined by microplate cultivations with online monitoring of biomass in a chemically defined medium and systematically omitting or substituting medium components. Persisting growth limitations were also detected, allowing further improvement of the chemically defined medium by the addition of glutamate group amino acids. Common complex media based on peptone and yeast extract were supplemented based on these findings. Optical density at the end of each cultivation of the improved peptone and yeast extract media roughly increased fivefold respectively. A maximum OD600 of 26.6 ± 0.7 (CDW: 17.1 ± 0.5 g/L) was reached with the improved yeast extract medium. Finally, culture performance and media improvement was analysed by measuring the oxygen transfer rate as well as the backscatter during shake flask cultivation.
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