ArticleHorticulture research2021
The transcription factor OpWRKY2 positively regulates the biosynthesis of the anticancer drug camptothecin in Ophiorrhiza pumila.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Cell-Specific Expression and Cellular Compartmental Regulation in Camptothecin Biosynthesis.Plant biotechnology journal · 2026Article
- The genome of the medicinal plant Uncaria rhynchophylla provides new insights into monoterpenoid indole alkaloid metabolism and its molecular regulatory mechanism.Molecular horticulture · 2026Article
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- Hepatocytes as Model for Investigating Natural Senotherapeutic Compounds and Their Effects on Cell Cycle Dynamics and Genome Stability.International journal of molecular sciences · 2025Review
- Targeted metabolic engineering of key biosynthetic genes improves triptolide production in Tripterygium wilfordii hairy roots.Plant cell reports · 2025Article
- Advances in WRKY regulation of immune responses in medicinal plants.Frontiers in plant science · 2025Review
- A transcription factor of SHI family AaSHI1 activates artemisinin biosynthesis genes in Artemisia annua.BMC genomics · 2024Article
- Biotechnological approaches for the production of camptothecin.Applied microbiology and biotechnology · 2024Review
- Genome-Wide Identification and Expression Analysis of WRKY Gene Family inInternational journal of molecular sciences · 2023Article
- Genomic profiling of WRKY transcription factors and functional analysis of CcWRKY7, CcWRKY29, and CcWRKY32 related to protoberberine alkaloids biosynthesis inFrontiers in genetics · 2023Article
- Profiles of Metabolic Genes inBiomolecules · 2022Article
- Interplay of transcription factors orchestrating the biosynthesis of plant alkaloids.3 Biotech · 2022Review
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila.BMC genomics · 2022Article
- Identification of WRKY transcription factors involved in regulating the biosynthesis of the anti-cancer drug camptothecin inHorticulture research · 2022Article
- Environmental and Genetic Factors Involved in Plant Protection-Associated Secondary Metabolite Biosynthesis Pathways.Frontiers in plant science · 2022Review
- Jasmonate-Responsive Transcription Factors NnWRKY70a and NnWRKY70b Positively Regulate Benzylisoquinoline Alkaloid Biosynthesis in Lotus (Frontiers in plant science · 2022Article
- Functional analysis ofFrontiers in plant science · 2022Article
- Plant-based expression platforms to produce high-value metabolites and proteins.Frontiers in plant science · 2022Review
- Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry. An example of this is camptothecin, a monoterpene indole alkaloid with potent anticancer activity that is extracted at very low concentrations from woody plants. Recently, camptothecin biosynthesis has been shown to become biotechnologically amenable in hairy-root systems of the natural producer Ophiorrhiza pumila. Here, time-course expression and metabolite analyses were performed to identify novel transcriptional regulators of camptothecin biosynthesis in O. pumila. It is shown here that camptothecin production increased over cultivation time and that the expression pattern of the WRKY transcription factor encoding gene OpWRKY2 is closely correlated with camptothecin accumulation. Overexpression of OpWRKY2 led to a more than three-fold increase in camptothecin levels. Accordingly, silencing of OpWRKY2 correlated with decreased camptothecin levels in the plant. Further detailed molecular characterization by electrophoretic mobility shift, yeast one-hybrid and dual-luciferase assays showed that OpWRKY2 directly binds and activates the central camptothecin pathway gene OpTDC. Taken together, the results of this study demonstrate that OpWRKY2 acts as a direct positive regulator of camptothecin biosynthesis. As such, a feasible strategy for the over-accumulation of camptothecin in a biotechnologically amenable system is presented.
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