Evidence map›Paper›PMID 33382976›Full record

ArticleStem cell reports2021

AP-2γ Is Required for Maintenance of Multipotent Mammary Stem Cells.

Vivian W Gu, Edward Cho, Dakota T Thompson, Victoria C Cassady, Nicholas Borcherding, Kelsey E Koch, Vincent T Wu, Allison W Lorenzen, Dana M van der Heide, Jeffrey R White and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Vivian W GuDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA; Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA.
Edward ChoDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Dakota T ThompsonDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Victoria C CassadyDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Nicholas BorcherdingDepartment of Pathology, University of Iowa, Iowa City, IA 52242, USA.
Kelsey E KochDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Vincent T WuDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Allison W LorenzenDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Dana M van der HeideDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Jeffrey R WhiteDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Mikhail V KulakDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA.
Trevor WilliamsDepartment of Craniofacial Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Weizhou ZhangDepartment of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA. Electronic address: zhangw@ufl.edu.
Ronald J WeigelDepartment of Surgery, University of Iowa, 200 Hawkins Drive, JCP 1509 Iowa City, IA 52242-1086, USA; Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA; Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA. Electronic address: ronald-weigel@uiowa.edu.
University of Iowa · USUniversity of Colorado Anschutz Medical Campus · USUniversity of Florida · US

Funding

Viral VectorP30CA086862 · NCI · UNIVERSITY OF IOWA · PI Christopher K Laubenthal · 2000 to 2026
$70.0M
MEDICAL SCIENTIST TRAINING PROGRAMT32GM007337 · NIGMS · UNIVERSITY OF IOWA · PI GEYER, PAMELA K., LENTZ, STEVEN R · 1985 to 2020
$15.9M
University of Iowa Surgical Oncology Training GrantT32CA148062 · NCI · UNIVERSITY OF IOWA · PI WEIGEL, RONALD J · 2010 to 2020
$3.8M
Obesity, inflammation and breast cancerR01CA200673 · NCI · UNIVERSITY OF IOWA · PI ZHANG, WEIZHOU · 2016 to 2020
$1.7M
CD177 suppresses breast-cancer development by inhibiting beta-cateninR01CA203834 · NCI · UNIVERSITY OF IOWA · PI ZHANG, WEIZHOU · 2016 to 2020
$1.7M
RET as a novel therapeutic target for breast cancerR01CA183702 · NCI · UNIVERSITY OF IOWA · PI WEIGEL, RONALD J · 2015 to 2019
$1.7M
The role of paracrine WNT5A/RYK in ErbB2-driven tumorigenesisF30CA206255 · NCI · UNIVERSITY OF IOWA · PI BORCHERDING, NICHOLAS · 2017 to 2020
$143k
NCI NIH HHS F30 CA206255NCI NIH HHS R01 CA183702NCI NIH HHS R01 CA200673NCI NIH HHS R01 CA203834NCI NIH HHS T32 CA148062NIGMS NIH HHS T32 GM007337
6 · The paper itself

Abstract

Mammary gland ductal morphogenesis depends on the differentiation of mammary stem cells (MaSCs) into basal and luminal lineages. The AP-2γ transcription factor, encoded by Tfap2c, has a central role in mammary gland development but its effect in mammary lineages and specifically MaSCs is largely unknown. Here, we utilized an inducible, conditional knockout of Tfap2c to elucidate the role of AP-2γ in maintenance and differentiation of MaSCs. Loss of AP-2γ in the basal epithelium profoundly altered the transcriptomes and decreased the number of cells within several clusters of mammary epithelial cells, including adult MaSCs and luminal progenitors. AP-2γ regulated the expression of genes known to be required for mammary development, including Cebpb, Nfkbia, and Rspo1. As a result, AP-2γ-deficient mice exhibited repressed mammary gland ductal outgrowth and inhibition of regenerative capacity. The findings demonstrate that AP-2γ can regulate development of mammary gland structures potentially regulating maintenance and differentiation of multipotent MaSCs.

Indexed as

AnimalsCCAAT-Enhancer-Binding Protein-betaFemaleGene Expression Regulation, DevelopmentalMammary Glands, AnimalMiceMice, KnockoutMultipotent Stem CellsNF-KappaB Inhibitor alphaRegenerationSequence Analysis, RNASingle-Cell AnalysisThrombospondinsTranscription Factor AP-2CCAAT-Enhancer-Binding Protein-betaCebpb protein, mouseNF-KappaB Inhibitor alphaNfkbia protein, mouseRSPO1 protein, mouseThrombospondinsTranscription Factor AP-2AP-2γdevelopmentmammary glandTfap2c

Identifiers

PMID33382976
PMCPMC7897584
OpenAlexW3113573290

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.