ReviewBiomicrofluidics2020
Enhanced single-cell encapsulation in microfluidic devices: From droplet generation to single-cell analysis.
Review in Biomicrofluidics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Streamlined stable CHO cell line development using droplet microfluidics with image-based monoclonality assessment.Bioprocess and biosystems engineering · 2026Article
- Capillary microfluidics aided single-cell manipulation for optical detection of bacterial pathogens.Biophysical reviews · 2026Review
- Harnessing droplet microfluidics and morphology-based deep learning for the label-free study of polymicrobial-phage interactions.Communications biology · 2025Article
- Droplet-based single-cell RNA sequencing: decoding cellular heterogeneity for breakthroughs in cancer, reproduction, and beyond.Journal of translational medicine · 2025Review
- Droplet Generation and Manipulation in Microfluidics: A Comprehensive Overview of Passive and Active Strategies.Biosensors · 2025Review
- Viscoelasticity of ECM and cells-origin, measurement and correlation.Mechanobiology in medicine · 2024Review
- A Simple Pump-Free Approach to Generating High-Throughput Microdroplets Using Oscillating Microcone Arrays.Micromachines · 2024Article
- A simple guideline for designing droplet microfluidic chips to achieve an improved single (bio)particle encapsulation rate using a stratified flow-assisted particle ordering method.Biomicrofluidics · 2024Article
- Deterministic Single-Cell Encapsulation in PEG Norbornene Microgels for Promoting Anti-Inflammatory Response and Therapeutic Delivery of Mesenchymal Stromal Cells.Advanced healthcare materials · 2024Article
- 3D printing in microfluidics: experimental optimization of droplet size and generation time through flow focusing, phase, and geometry variation.RSC advances · 2024Article
- The use of droplet-based microfluidic technologies for accelerated selection ofBiology methods & protocols · 2024Review
- Towards single cell encapsulation for precision biology and medicine.Advanced drug delivery reviews · 2023Review
- Selective expansion of target cells using the Enrich TROVO platform.BioTechniques · 2023Article
- A home-made pipette droplet microfluidics rapid prototyping and training kit for digital PCR, microorganism/cell encapsulation and controlled microgel synthesis.Scientific reports · 2023Article
- Droplets microfluidics platform-A tool for single cell research.Frontiers in bioengineering and biotechnology · 2023Review
- A Dual-Channel Microfluidic Chip for Single Tobacco Protoplast Isolation and Dynamic Capture.Micromachines · 2022Article
- Single-Cell Microgels for Diagnostics and Therapeutics.Advanced functional materials · 2021Article
- Dual dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing.Lab on a chip · 2021Article
- Encapsulated Cell Dynamics in Droplet Microfluidic Devices with Sheath Flow.Micromachines · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell analysis to investigate cellular heterogeneity and cell-to-cell interactions is a crucial compartment to answer key questions in important biological mechanisms. Droplet-based microfluidics appears to be the ideal platform for such a purpose because the compartmentalization of single cells into microdroplets offers unique advantages of enhancing assay sensitivity, protecting cells against external stresses, allowing versatile and precise manipulations over tested samples, and providing a stable microenvironment for long-term cell proliferation and observation. The present Review aims to give a preliminary guidance for researchers from different backgrounds to explore the field of single-cell encapsulation and analysis. A comprehensive and introductory overview of the droplet formation mechanism, fabrication methods of microchips, and a myriad of passive and active encapsulation techniques to enhance single-cell encapsulation efficiency were presented. Meanwhile, common methods for single-cell analysis, especially for long-term cell proliferation, differentiation, and observation inside microcapsules, are briefly introduced. Finally, the major challenges faced in the field are illustrated, and potential prospects for future work are discussed.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.