ArticleThe Korean journal of pain2021
The proper concentrations of dextrose and lidocaine in regenerative injection therapy:
Article in The Korean journal of pain, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Dextrose prolotherapyeffectin improving thetemporomandibular joint disc displacement symptoms without reduction refractory to conservative treatment: a pilot study.Scientific reports · 2025Trial
- Evaluation of the effectiveness of dextrose injection in improving chewing function in patients with the temporomandibular joint closed lock: a pilot study.Oral and maxillofacial surgery · 2025Trial
- Microneedle Therapy System Combined with Glucose 10% for the Treatment of Enlarged Pores and Fine Wrinkles.Aesthetic plastic surgery · 2026Observational
- Patient-Specific Dual-Function 3D-Printed Gels for Antimicrobial and Analgesic Management of Alveolar Osteitis.ACS materials Au · 2026Article
- Article
- Sweet relief: the role of glucopuncture in neuropathic pain management.The Korean journal of pain · 2026Review
- Rapid Decrease in Dextrose Concentration After Intra-Articular Knee Injection: Implications for Mechanism of Action of Dextrose Prolotherapy.Biomedicines · 2025Article
- Prolotherapy in the Treatment of Sports-Related Tendinopathies: A Systematic Review of Randomized Controlled Trials.Orthopaedic journal of sports medicine · 2024Review
- Article
- The Potential of Glucose Treatment to Reduce Reactive Oxygen Species Production and Apoptosis of Inflamed Neural Cells In Vitro.Biomedicines · 2023Article
- Article
- A Comprehensive Update of Prolotherapy in the Management of Osteoarthritis of the Knee.Orthopedic reviews · 2022Article
- Perineural local anesthetic treatments for osteoarthritic pain.Regenerative engineering and translational medicine · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProlotherapy is a proliferation therapy as an alternative medicine. A combination of dextrose solution and lidocaine is usually used in prolotherapy. The concentrations of dextrose and lidocaine used in the clinical field are very high (dextrose 10%-25%, lidocaine 0.075%-1%). Several studies show about 1% dextrose and more than 0.2% lidocaine induced cell death in various cell types. We investigated the effects of low concentrations of dextrose and lidocaine in fibroblasts and suggest the optimal range of concentrations of dextrose and lidocaine in prolotherapy.
methodsVarious concentrations of dextrose and lidocaine were treated in NIH-3T3. Viability was examined with trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Migration assay was performed for measuring the motile activity. Extracellular signal-regulated kinase (Erk) activation and protein expression of collagen I and α-smooth muscle actin (α-SMA) were determined with western blot analysis.
resultsThe cell viability was decreased in concentrations of more than 5% dextrose and 0.1% lidocaine. However, in the concentrations 1% dextrose (D1) and 0.01% lidocaine (L0.01), fibroblasts proliferated mildly. The ability of migration in fibroblast was increased in the D1, L0.01, and D1 + L0.01 groups sequentially. D1 and L0.01 increased Erk activation and the expression of collagen I and α-SMA and D1 + L0.01 further increased. The inhibition of Erk activation suppressed fibroblast proliferation and the synthesis of collagen I.
conclusionsD1, L0.01, and the combination of D1 and L0.01 induced fibroblast proliferation and increased collagen I synthesis
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