Evidence map›Paper›PMID 33380386›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2020

[ALKBH5 suppresses migration and invasion of human trophoblast cells by inhibiting epithelial-mesenchymal transition].

Jianping He, Xiaojuan Li, Mengxin Lü, Jue Wang, Jian Tang, Shengjun Luo, Yuan Qian

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. N6-methyladenosine (m6A) Modification in Preeclampsia.Reproductive sciences (Thousand Oaks, Calif.) · 2023
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Jianping HeDepartment of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Healthcare Hospital, Kunming 650031, China.
Xiaojuan LiDepartment of Obstetrics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
Mengxin LüDepartment of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Healthcare Hospital, Kunming 650031, China.
Jue WangPrenatal Diagnosis Room, Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
Jian TangDepartment of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Healthcare Hospital, Kunming 650031, China.
Shengjun LuoDepartment of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Healthcare Hospital, Kunming 650031, China.
Yuan QianDepartment of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Healthcare Hospital, Kunming 650031, China.
Yunnan Maternal and Child Health · CNKunming Medical University · CNYunnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effects of ALKBH5 on migration, invasion and epithelial-mesenchymal transition (EMT) of human trophoblast cells.

methodsThe expression plasmid of ALKBH5 or a negative control plasmid (ALKBH5-NC) was transfected in human trophoblast HTR-8 /SVneo cells, and the expressions of ALKBH5 mRNA and protein were detected by qRT-PCR and Western blotting. Transwell assay was used to assess the changes in migration and invasion abilities of the trophoblast cells after the transfection. Western blotting was performed to detect the expressions of EMT-related proteins in the cells including vimentin, fibronectin, E-cadherin, N-cadherin, MMP9 and MMP2.

resultsALKBH5 mRNA and protein expressions were significantly higher in ALKBH5 group than in the control group (

conclusionsALKBH5 is involved in the pathogenesis of preeclampsia by inhibiting EMT of trophoblast cells and hence reducing their migration and invasion abilities.

Indexed as

Pre-EclampsiaTrophoblastsAlkB Homolog 5, RNA DemethylaseCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionFemaleHumansPregnancyVimentinALKBH5 protein, humanAlkB Homolog 5, RNA DemethylaseVimentinALKBH5epithelial-mesenchymal transitioninvasionmigrationpreeclampsia

Identifiers

PMID33380386
PMCPMC7835694
OpenAlexW3115163408

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.