Evidence map›Paper›PMID 33377101›Full record

ArticleSTAR protocols2020

Purification and Use of tRNA for Enzymatic Post-translational Addition of Amino Acids to Proteins.

Irem Avcilar-Kucukgoze, Howard Gamper, Ya-Ming Hou, Anna Kashina

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 25 citations in OpenAlex.

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  17. Synthesis of Peptides and Proteins with Site-Specific Glutamate Arginylation.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Irem Avcilar-KucukgozeDepartment of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Howard GamperDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19144, USA.
Ya-Ming HouDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19144, USA.
Anna KashinaDepartment of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Thomas Jefferson University · USUniversity of Pennsylvania · US

Funding

Regulation of cell migration by nucleotide coding sequence and arginylationR35GM122505 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Anna S Kashina · 2017 to 2026
$6.2M
tRNA in codon usageR35GM134931 · NIGMS · THOMAS JEFFERSON UNIVERSITY · PI HOU, YA-MING · 2020 to 2025
$5.4M
Role of arginylation in prevention of alpha synuclein-driven neurodegenerationR01NS102435 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI KASHINA, ANNA S · 2019 to 2023
$2.6M
Discovery of Gram-negative permeable chemical probes for tRNA methylationR01AI139202 · NIAID · THOMAS JEFFERSON UNIVERSITY · PI HOU, YA-MING · 2019 to 2022
$2.4M
Probe discovery for tRNA methylationR01GM126210 · NIGMS · THOMAS JEFFERSON UNIVERSITY · PI HOU, YA-MING, SMITH, LAYTON HARRIS · 2018 to 2020
$1.2M
Mining the tRNA genome by live-cell imagingR21HG010824 · NHGRI · THOMAS JEFFERSON UNIVERSITY · PI AHERN, CHRISTOPHER A, HOU, YA-MING · 2019 to 2020
$441k
NHGRI NIH HHS R21 HG010824NIAID NIH HHS R01 AI139202NIGMS NIH HHS R01 GM126210NIGMS NIH HHS R35 GM122505NIGMS NIH HHS R35 GM134931NINDS NIH HHS R01 NS102435
6 · The paper itself

Abstract

Post-translational addition of amino acids to proteins by enzymes using aminoacyl-tRNA is an emerging regulatory mechanism. Examples include Arg transfer in eukaryotes, Leu/Phe transfer in bacteria, and tRNA-synthetase-mediated addition of amino acids to Lys side chains. Here, we present a method of purification and use of tRNA for such reactions, focusing on tRNA

Indexed as

Amino AcidsAmino Acyl-tRNA SynthetasesArginineEnzyme AssaysProtein Processing, Post-TranslationalRNA, TransferRNA, Transfer, Amino AcylTranscription, GeneticAmino AcidsAmino Acyl-tRNA SynthetasesArginineRNA, TransferRNA, Transfer, Amino AcylMolecular BiologyMolecular/Chemical ProbesProtein Biochemistry

Identifiers

PMID33377101
PMCPMC7757669
OpenAlexW3110951245

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.