Evidence map›Paper›PMID 33377097›Full record

ArticleSTAR protocols2020

Visualizing Cellular Dynamics and Protein Localization in 3D Collagen.

Wan Hon Koh, Romaniya Zayats, Paul Lopez, Thomas T Murooka

Abstract read
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Wan Hon KohUniversity of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, Canada.
Romaniya ZayatsUniversity of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, Canada.
Paul LopezUniversity of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, Canada.
Thomas T MurookaUniversity of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, Canada.

Funding

CIHR HB2 164064CIHR PJT-155951CIHR XGG-142962
6 · The paper itself

Abstract

Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response. While studying these events in cell culture are standard procedure, spatiotemporal dynamics of cell-to-cell interactions within three-dimensional (3D) environments are critical in generating appropriate effector functions. Here, we present a detailed protocol to study cells within an all-in-one 3D collagen matrix that is amenable to live-cell microscopy and immunohistochemistry. This approach facilitates analyses of dynamic cellular events in 3D settings. For complete details on the use and execution of this protocol, please refer to Koh et al. (2020).

Indexed as

Biochemical PhenomenaCell CommunicationCell Culture TechniquesCell Culture Techniques, Three DimensionalCell MovementCollagenCytokinesHumansMicroscopyProtein TransportCollagenCytokinesCell-based AssaysCell BiologyImmunologyMicroscopy

Identifiers

PMID33377097
PMCPMC7757667

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.