Evidence map›Paper›PMID 33377093›Full record

ArticleSTAR protocols2020

Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR).

David Rincon Fernandez Pacheco, Sara Sabet, Joshua J Breunig

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. STAR protocols · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

David Rincon Fernandez PachecoBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Sara SabetBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Joshua J BreunigBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Cedars-Sinai Medical Center · US

Funding

A Genetic Toolbox for the Identification and Interrogation of Tumor Cells of Origin, Propagation, and Recurrence From Patient-derived Oncogenic DriversR33CA202900 · NCI · CEDARS-SINAI MEDICAL CENTER · PI BREUNIG, JOSHUA JOHN · 2016 to 2018
$1.2M
GESTALT Barcoding and Single-cell Transcriptomics of Tumor Cell Evolution in Personalized Tumor ModelsR33CA236687 · NCI · CEDARS-SINAI MEDICAL CENTER · PI BREUNIG, JOSHUA JOHN · 2019 to 2021
$1.2M
Direct Differentiations of iPSCs: an Autologous and Scalable Source of Striatal Medium Spiny Neurons for Cellular Transplantation TherapyR03NS101529 · NINDS · CEDARS-SINAI MEDICAL CENTER · PI BREUNIG, JOSHUA JOHN · 2017 to 2018
$175k
NCI NIH HHS R33 CA202900NCI NIH HHS R33 CA236687NINDS NIH HHS R03 NS101529
6 · The paper itself

Abstract

This protocol focuses on the cloning and stable integration of sequences of interest by the use of a mosaic analysis with dual recombinases (MADR) plasmid that includes fusion proteins or independent proteins under the control of 2A peptide or IRES elements. Additionally, we describe how to generate a neural stem cell culture from Gt(ROSA)26Sort

Indexed as

AnimalsAnimals, Genetically ModifiedElectroporationGenetic EngineeringGenetic VectorsGreen Fluorescent ProteinsLuminescent ProteinsMicePlasmidsRecombinasesRed Fluorescent ProteinTransfectionGreen Fluorescent ProteinsLuminescent ProteinsRecombinasesRed Fluorescent ProteinCRISPRGene ExpressionModel OrganismsMolecular BiologyNeuroscience

Identifiers

PMID33377093
PMCPMC7757563
OpenAlexW3112258966

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.