Evidence map›Paper›PMID 33377076›Full record

ArticleSTAR protocols2020

A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates.

Andrey A Petropavlovskiy, Michael G Tauro, Patrick Lajoie, Martin L Duennwald

Abstract read
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Applying nephelometry for analyzing liquid yeast cultures.Biochemistry and biophysics reports · 2026
    Article
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  7. Cytoplasmic circular dsDNA is a key constituent of stress granules.bioRxiv : the preprint server for biology · 2026
    Article
  8. Article
  9. Article
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  11. Article
  12. Article
  13. A neuregulin-like ligand and EGF receptor underpinFrontiers in cellular and infection microbiology · 2026
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. An N-terminal domain specifies developmental control by the SMAX1-LIKE family of transcriptional regulators inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Andrey A PetropavlovskiyDepartment of Biology, The University of Western Ontario, London, ON N6A 3K7, Canada.
Michael G TauroDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada.
Patrick LajoieDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada.
Martin L DuennwaldDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada.

Funding

CIHR 325538
6 · The paper itself

Abstract

We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike. For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020).

Indexed as

Imaging, Three-DimensionalMicrobial ViabilityAgarAlgorithmsCell Culture TechniquesExonsHuntingtin ProteinSaccharomyces cerevisiaeAgarHuntingtin ProteinCell BiologyModel OrganismsMolecular Biology

Identifiers

PMID33377076
PMCPMC7757406

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.