Evidence map›Paper›PMID 33377052›Full record

ArticleSTAR protocols2020

Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells.

Haibin Xi, Courtney S Young, April D Pyle

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Haibin XiDepartment of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA.
Courtney S YoungMyoGene Bio, Los Angeles, CA 90024, USA.
April D PyleDepartment of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA.
University of California, Los Angeles · US

Funding

Reprogramming and Directed Differentiation of Skeletal Muscle Cells from hPSCs.R01AR064327 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI April D Pyle · 2013 to 2026
$6.4M
NIAMS NIH HHS R01 AR064327
6 · The paper itself

Abstract

This protocol describes the use of CRISPR/Cas9-mediated homology-directed recombination to construct a PAX7-GFP reporter in human pluripotent stem cells (hPSCs). PAX7 is a key transcription factor and regulator of skeletal muscle stem/progenitor cells. We obtained heterozygous knockin reporter cells and validated their PAX7 expression using both artificial activation by the CRISPR/dCas9-VPR system and physiological activation during hPSC myogenic differentiation. These cells can serve as tools for better understanding of

Indexed as

Genes, ReporterMuscle Development3' Untranslated RegionsAmino Acid SequenceAnimalsBase SequenceBinding SitesCell CountCell DifferentiationConserved SequenceCRISPR-Associated Protein 9CRISPR-Cas SystemsDrug Resistance, MicrobialGenotypeHumansMammals3' Untranslated RegionsCRISPR-Associated Protein 9MicroRNAsPAX7 Transcription FactorProtein IsoformsRNA, Guide, CRISPR-Cas Systems

Identifiers

PMID33377052
PMCPMC7757361
OpenAlexW3097437698

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.