Evidence map›Paper›PMID 33377049›Full record

ArticleSTAR protocols2020

Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2.

Jiuyang Liu, Zhaoyu Xue, Kendra R Vann, Xiaobing Shi, Tatiana G Kutateladze

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jiuyang LiuDepartment of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Zhaoyu XueCenter for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
Kendra R VannDepartment of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Xiaobing ShiCenter for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
Tatiana G KutateladzeDepartment of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.

Funding

Role of GAS41 in Human CancerR01CA204020 · NCI · VAN ANDEL RESEARCH INSTITUTE · PI SHI, XIAOBING · 2016 to 2020
$2.0M
Molecular analysis of ASH1LR01CA252707 · NCI · UNIVERSITY OF COLORADO DENVER · PI TATIANA G KUTATELADZE · 2020 to 2026
$1.9M
The role of JADE in HBO complexesR01HL151334 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI KUTATELADZE, TATIANA G · 2020 to 2023
$1.8M
Epigenetic mechanisms for regulation of p300R01GM135671 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI KUTATELADZE, TATIANA G · 2020 to 2023
$1.4M
Interdomain crosstalk in MORC3R01GM125195 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI KUTATELADZE, TATIANA G · 2017 to 2021
$1.3M
NCI NIH HHS R01 CA204020NCI NIH HHS R01 CA252707NHLBI NIH HHS R01 HL151334NIGMS NIH HHS R01 GM125195NIGMS NIH HHS R01 GM135671
6 · The paper itself

Abstract

Since its discovery, several ligands of the ZZ domain have been identified; however, molecular and structural information underlying binding of these ligands remains limited. Here, we describe a protocol for biochemical and structural analysis of the ZZ domain of human E3 ubiquitin ligase HERC2 (HERC2

Indexed as

BiochemistryBuffersCrystallizationFluorescenceHEK293 CellsHistonesHumansMagnetic Resonance SpectroscopyPeptidesProtein DomainsRecombinant Fusion ProteinsSUMO-1 ProteinTryptophanUbiquitin-Protein LigasesBuffersHERC2 protein, humanHistonesPeptidesRecombinant Fusion ProteinsSUMO-1 ProteinSUMO1 protein, humanTryptophanUbiquitin-Protein Ligases

Identifiers

PMID33377049
PMCPMC7757301

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.