ArticleSTAR protocols2020
Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes.
Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Adipocyte Myoglobin Is a Determinant of Energy Expenditure and a Potential Target to Limit Obesity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Exploring Adipose Tissue Complexity Through Omics Approaches: Implications for Health and Disease.Cells · 2026Review
- A distal enhancer of Pparα regulates thermogenesis and mitochondrial function in brown fat.PLoS genetics · 2025Article
- Identification of Neuritin 1 as a local metabolic regulator of brown adipose tissue.Nature communications · 2025Article
- Sex-specific changes in energy demand during the preplaque stage in a transgenic Alzheimer's mouse model.Biology of sex differences · 2025Article
- Intracellular Sphingosine-1-Phosphate Induces Lipolysis Through Direct Activation of Protein Kinase C Zeta.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Expression of p53 in human adipose tissue correlates positively with FAS and BMI.International journal of obesity (2005) · 2025Article
- Identification of a distal enhancer of Ucp1 essential for thermogenesis and mitochondrial function in brown fat.Communications biology · 2025Article
- Development of a functional beige fat cell line uncovers independent subclasses of cells expressing UCP1 and the futile creatine cycle.Cell metabolism · 2024Article
- Mitochondrial lipidomes are tissue specific - low cholesterol contents relate to UCP1 activity.Life science alliance · 2024Article
- Lipid-associated macrophages reshape BAT cell identity in obesity.Cell reports · 2024Article
- Primary adipocytes as targetable drug depot to prevent post-surgical cancer recurrence.Materials today. Bio · 2024Article
- Adipocyte retinoic acid receptor α prevents obesity and steatohepatitis by regulating energy expenditure and lipogenesis.Obesity (Silver Spring, Md.) · 2024Article
- Optogenetics Sheds Light on Brown and Beige Adipocytes.Journal of cellular signaling · 2023Article
- Dolutegravir Suppresses Thermogenesis via Disrupting Uncoupling Protein 1 Expression and Mitochondrial Function in Brown/Beige Adipocytes in Preclinical Models.The Journal of infectious diseases · 2022Article
- Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat.Molecular metabolism · 2022Article
- Adipocyte HIF2α functions as a thermostat via PKA Cα regulation in beige adipocytes.Nature communications · 2022Article
- Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.Cell metabolism · 2022Article
- RNA-Seq reveals miRNA role in thermogenic regulation in brown adipose tissues of goats.BMC genomics · 2022Article
- Methionine adenosyltransferase 1a antisense oligonucleotides activate the liver-brown adipose tissue axis preventing obesity and associated hepatosteatosis.Nature communications · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying brown and brite adipose tissue requires precise and reliable quantification of cellular thermogenesis. This protocol describes the isolation of primary murine pre-adipocytes, differentiation into thermogenic brown and brite adipocytes, and subsequent oxygen consumption analysis. Commonly applied procedures only measure basal and maximal proton leak-linked oxygen consumption but not explicitly uncoupling protein 1 (UCP1)-dependent respiration. Meaningful oxygen consumption analyses require (1) the activation of UCP1, (2) control over intracellular free-fatty-acid levels, and (3) inhibition of ATP-consuming futile cycles. For complete details on the use and execution of this protocol, please refer to Li et al. (2014, 2017, 2018) and Schweizer et al. (2018).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.