Evidence map›Paper›PMID 33377006›Full record

ArticleSTAR protocols2020

An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root.

Goh Choe, Nam V Hoang, Ji-Young Lee

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Goh ChoeSchool of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Nam V HoangSchool of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Ji-Young LeeSchool of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Seoul National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Laser capture microdissection (LCM) coupled with transcriptome profiling is a powerful technique that allows for tissue-specific gene expression analysis in a complex system. One major challenge in using this technique is to obtain RNA without compromising its integrity. Here, we present a protocol optimized for radish root tissue sections using Steedman's wax embedding to obtain high-quality RNA suitable for next-generation sequencing analysis. For complete details on the use and execution of this protocol, please refer to Hoang et al. (2020).

Indexed as

Gene ExpressionGene Expression ProfilingHigh-Throughput Nucleotide SequencingLaser Capture MicrodissectionOrgan SpecificityPlant RootsRaphanusRNATranscriptomeRNA

Identifiers

PMID33377006
PMCPMC7756924
OpenAlexW3087636562

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.