ArticleiScience2020
RNAseq Analysis of Rodent Spaceflight Experiments Is Confounded by Sample Collection Techniques.
Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Commentary: a review of technical considerations for planning an RNA-Sequencing experiment.BMC genomics · 2025Review
- Mission SpaceX CRS-16 RRRM-1 space flight induced skin genomic plasticity via an epigenetic trigger.iScience · 2024Article
- Microgravity's effects on miRNA-mRNA regulatory networks in a mouse model of segmental bone defects.PloS one · 2024Article
- Meta-analysis of the space flight and microgravity response of the Arabidopsis plant transcriptome.NPJ microgravity · 2023Article
- Challenges and considerations for single-cell and spatially resolved transcriptomics sample collection during spaceflight.Cell reports methods · 2022Review
- Acute stress induces severe neural inflammation and overactivation of glucocorticoid signaling in interleukin-18-deficient mice.Translational psychiatry · 2022Article
- Artificial gravity partially protects space-induced neurological deficits in Drosophila melanogaster.Cell reports · 2022Article
- Rad-Bio-App: a discovery environment for biologists to explore spaceflight-related radiation exposures.NPJ microgravity · 2021Article
- NASA GeneLab RNA-seq consensus pipeline: standardized processing of short-read RNA-seq data.iScience · 2021Article
- A New Era for Space Life Science: International Standards for Space Omics Processing.Patterns (New York, N.Y.) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the physiological changes that occur in response to spaceflight, mice are transported to the International Space Station (ISS) and housed for variable periods of time before euthanasia on-orbit or return to Earth. Sample collection under such difficult conditions introduces confounding factors that need to be identified and addressed. We found large changes in the transcriptome of mouse tissues dissected and preserved on-orbit compared with tissues from mice euthanized on-orbit, preserved, and dissected after return to Earth. Changes due to preservation method eclipsed those between flight and ground samples, making it difficult to identify spaceflight-specific changes. Follow-on experiments to interrogate the roles of euthanasia methods, tissue and carcass preservation protocols, and library preparation methods suggested that differences due to preservation protocols are exacerbated when coupled with polyA selection. This has important implications for the interpretation of existing datasets and the design of future experiments.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.