ArticleVeterinary medicine international2020
Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Aba'ala District of Afar Region, Ethiopia.
Article in Veterinary medicine international, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Evolutionary and Spatiotemporal Dynamics of Foot-and-Mouth Disease Virus in Ethiopia (1957-2025): A Molecular Perspective on Serotype Persistence and Emergence.Transboundary and emerging diseases · 2026Review
- Comparison of two continuous cell lines BHK-21 and IB-IS2 for isolating field serotypes O and A of Foot-and-Mouth disease virus.Virus genes · 2025Article
- The isolation and serotyping of foot-and-mouth disease virus in Iran during 2019-2022.Virus genes · 2025Article
- Seroprevalence and molecular detection of foot and mouth disease virus in cattle in selected districts of Wolaita Zone, Southern Ethiopia.Scientific reports · 2024Article
- One Health Paradigm to Confront Zoonotic Health Threats: A Pakistan Prospective.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOn the basis of FMDV outbreak cases, a cross-sectional study was undertaken to collect samples from January 2019 to March 2020 intended for isolation, serotyping, and molecular detection of FMDV in the study district. The purposive sampling method was applied to select the study area for the reason of the presence of FMD outbreak case report during the study period. Totally, 27 FMD clinical samples were collected from affected study population during field outbreak. Out of 27 samples, 18 of them were inoculated on cultured Baby hamster kidney (BHK-21) monolayer cells, and all 27 samples were tested using conventional RT-PCR and sets of specific universal primers. Finally, the PCR products were visualized with UV illumination and imaged with gel documentation system.
resultsThe current study results revealed that out of 18 clinical samples subjected to virus isolation, 72.2% (
conclusionsOur study finding indicated that FMDV is prevalent in the study area and FMDV serotype SAT-2 was the causality for the outbreaks of the disease in the study area. Hence, region-wise FMD outbreak investigation, further phylogenetic analysis, and vaccine matching field isolates should be carried out for effective vaccine development to control the disease.
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