Evidence map›Paper›PMID 33376255›Full record

ArticleBiotechnology and applied biochemistry

Promoting P450 BM3 heme domain dimerization with a tris(5-iodoacetamido-1,10-phenanthroline)Ru(II) complex.

Mallory Kato, Bridget Foley, Julia Vu, Michael Huynh, Kathreena Lucero, Caroline Harmon, Lionel Cheruzel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and applied biochemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
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  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Mallory KatoSan José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101.
Bridget FoleySan José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101.
Julia VuSan José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101.
Michael HuynhSan José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101.
Kathreena LuceroSan José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101.
Caroline HarmonSan José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101.
Lionel CheruzelSan José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101.
San Jose State University · US

Funding

Ru(II)-diimine labeled P450 mutants for the selective hydroxylation of substrateSC3GM095415 · NIGMS · SAN JOSE STATE UNIVERSITY · PI CHERUZEL, LIONEL E. · 2011 to 2018
$860k
NIGMS NIH HHS SC3 GM095415
6 · The paper itself

Abstract

Protein dimerization often occurs in many biological systems as to provide structural and functional advantages. A tris(5-iodoacetamido-1,10-phenanthroline)Ruthenium(II) complex was shown to promote the covalent dimerization of a P450 BM3 heme domain mutant containing a surface exposed non-native single cysteine residue. The formation of homodimeric species was confirmed by protein gel electrophoresis, mass spectrometry and UV-Vis spectroscopy. The dimeric species could be separated from the monomer and aggregates by size-exclusion chromatography. Docking simulation reveals a plausible structure with two proteins covalently conjugated to the inorganic compound.

Indexed as

Protein MultimerizationAmino Acid SubstitutionCoordination ComplexesCytochrome P-450 Enzyme SystemHemeMutation, MissensePhenanthrolinesProtein DomainsRuthenium5-iodoacetamido-1,10-phenanthrolineCoordination ComplexesCytochrome P-450 Enzyme SystemHemePhenanthrolinesRuthenium

Identifiers

PMID33376255
PMCPMC7769117
OpenAlexW3036986571

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.