Evidence map›Paper›PMID 33376194›Full record

ArticleMolecular & cellular proteomics : MCP2021

Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MS

Simone Kurz, M Osman Sheikh, Shan Lu, Lance Wells, Michael Tiemeyer

Open access · hybridAbstract read
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
  2. Review
  3. Biomolecules · 2023
    Article
  4. Review
  5. LC-MS/MS Isomeric Profiling ofBiomolecules · 2022
    Article
  6. Article
  7. In-Depth Profiling ofAnalytical chemistry · 2022
    Article
  8. Review
  9. Review
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Simone KurzComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
M Osman SheikhComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
Shan LuDepartment of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Lance WellsComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA. Electronic address: lwells@ccrc.uga.edu.
Michael TiemeyerComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA. Electronic address: mtiemeyer@ccrc.uga.edu.
University of Georgia · USUniversity of Massachusetts Chan Medical School · US

Funding

TR&D1: Stem Cell and Induced Pluripotent Stem Cell Resources (Pages 116-134)P41GM103490 · NIGMS · UNIVERSITY OF GEORGIA · PI PIERCE, J. MICHAEL · 2012 to 2018
$13.7M
Structure and Function in alpha-Dystroglycan GlycosylationR01GM111939 · NIGMS · UNIVERSITY OF GEORGIA · PI Lance Wells · 2014 to 2026
$4.0M
Origin of N-Glycan Site-Specific HeterogeneityR01GM130915 · NIGMS · UNIVERSITY OF GEORGIA · PI KANNAN, NATARAJAN, MOREMEN, KELLEY W. · 2019 to 2022
$3.4M
Enabling tools for protists pathogen glycobiologyR21AI123161 · NIAID · UNIVERSITY OF GEORGIA · PI TARLETON, RICK L, WELLS, LANCE · 2015 to 2016
$745k
NIAID NIH HHS R21 AI123161NIGMS NIH HHS P41 GM103490NIGMS NIH HHS R01 GM111939NIGMS NIH HHS R01 GM130915
6 · The paper itself

Abstract

HPLC has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures. Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods. These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments. A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MS

Indexed as

AnimalsBrainCell LineChromatography, Reverse-PhaseEpithelial CellsGlycomicsHumansIsomerismMethylationMicePolysaccharidesTandem Mass SpectrometryPolysaccharidesglycanion trapisomerliquid chromatographymass spectrometrynanoflownano spray ionizationpermethylationreverse phase

Identifiers

PMID33376194
PMCPMC8724860
OpenAlexW3115772304

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.