ArticleThe Journal of biological chemistry
Structure and mechanism of Staphylococcus aureus oleate hydratase (OhyA).
Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- A Comparative Biochemical Study of Oleate Hydratases.Chembiochem : a European journal of chemical biology · 2026Article
- Revealing the Fate of Isomeric Monounsaturated Fatty Acids inACS infectious diseases · 2026Article
- Biosensor Based on Electrochemical Analysis forPolymers · 2025Article
- A Thermostable Fatty Acid Hydratase from Marinitoga Piezophila with Low Temperature Optimum and Broad Product Scope.Chembiochem : a European journal of chemical biology · 2025Article
- Exploring Oxylipins in Host-Microbe Interactions and Their Impact on Infection and Immunity.Current issues in molecular biology · 2025Review
- Fatty acid composition in the vaginal tract of cis-gender women: canary in coal mines for reproductive health?Lipids in health and disease · 2025Review
- Convergent reductive evolution in bee-associated lactic acid bacteria.Applied and environmental microbiology · 2024Article
- Article
- Cryo-EM reconstruction of oleate hydratase bound to a phospholipid membrane bilayer.Journal of structural biology · 2024Article
- Convergent reductive evolution in bee-associated lactic acid bacteria.bioRxiv : the preprint server for biology · 2024Article
- Review
- Article
- Article
- Independent evolution of oleate hydratase clades in Bacillales reflects molecular convergence.Frontiers in molecular biosciences · 2024Article
- Dissecting the biophysical mechanisms of oleate hydratase association with membranes.Frontiers in molecular biosciences · 2024Article
- VaginalbioRxiv : the preprint server for biology · 2023Article
- Chiral Alcohols from Alkenes and Water: Directed Evolution of a Styrene Hydratase.Angewandte Chemie (International ed. in English) · 2023Article
- Towards an understanding of oleate hydratases and their application in industrial processes.Microbial cell factories · 2022Review
- Oleate Hydratase (OhyA) Is a Virulence Determinant in Staphylococcus aureus.Microbiology spectrum · 2021Article
- Novel oleate hydratases and potential biotechnological applications.Applied microbiology and biotechnology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Flavin adenine dinucleotide (FAD)-dependent bacterial oleate hydratases (OhyAs) catalyze the addition of water to isolated fatty acid carbon-carbon double bonds. Staphylococcus aureus uses OhyA to counteract the host innate immune response by inactivating antimicrobial unsaturated fatty acids. Mechanistic information explaining how OhyAs catalyze regiospecific and stereospecific hydration is required to understand their biological functions and the potential for engineering new products. In this study, we deduced the catalytic mechanism of OhyA from multiple structures of S. aureus OhyA in binary and ternary complexes with combinations of ligands along with biochemical analyses of relevant mutants. The substrate-free state shows Arg81 is the gatekeeper that controls fatty acid entrance to the active site. FAD binding engages the catalytic loop to simultaneously rotate Glu82 into its active conformation and Arg81 out of the hydrophobic substrate tunnel, allowing the fatty acid to rotate into the active site. FAD binding also dehydrates the active site, leaving a single water molecule connected to Glu82. This active site water is a hydronium ion based on the analysis of its hydrogen bond network in the OhyA•PEG400•FAD complex. We conclude that OhyA accelerates acid-catalyzed alkene hydration by positioning the fatty acid double bond to attack the active site hydronium ion, followed by the addition of water to the transient carbocation intermediate. Structural transitions within S. aureus OhyA channel oleate to the active site, curl oleate around the substrate water, and stabilize the hydroxylated product to inactivate antimicrobial fatty acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.