Evidence map›Paper›PMID 33374320›Full record

ArticleInternational journal of molecular sciences2020

FRCaMP, a Red Fluorescent Genetically Encoded Calcium Indicator Based on Calmodulin from Schizosaccharomyces Pombe Fungus.

Oksana M Subach, Natalia V Barykina, Elizaveta S Chefanova, Anna V Vlaskina, Vladimir P Sotskov, Olga I Ivashkina, Konstantin V Anokhin, Fedor V Subach

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.8field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. The GEM-GECO Calcium Indicator Is Useable inInternational journal of molecular sciences · 2022
    Article
  9. The mRubyFT Protein, Genetically Encoded Blue-to-Red Fluorescent Timer.International journal of molecular sciences · 2022
    Article
  10. Optogenetics for light control of biological systems.Nature reviews. Methods primers · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Oksana M SubachComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
Natalia V BarykinaLaboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology, 125315 Moscow, Russia.ORCID 0000-0001-5835-8388
Elizaveta S ChefanovaDepartment of NBIC-Technologies, Moscow Institute of Physics and Technology, 123182 Moscow, Russia.
Anna V VlaskinaComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
Vladimir P SotskovInstitute for Advanced Brain Studies, Lomonosov Moscow State University, 119991 Moscow, Russia.ORCID 0000-0002-1729-5744
Olga I IvashkinaComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
Konstantin V AnokhinLaboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology, 125315 Moscow, Russia.
Fedor V SubachComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.ORCID 0000-0003-2720-7821
Kurchatov Institute · RULomonosov Moscow State University · RUInstitute of Normal Physiology named after P.K. Anokhin · RUMoscow Institute of Physics and Technology · RU

Funding

internal grant of the National Research Center Kurchatov Institute №1056 от 02.07.2020Russian Foundation for Basic Research 19-04-00395the Ministry of Science and Higher Education of the Russian Federation 075-15-2020-801
6 · The paper itself

Abstract

Red fluorescent genetically encoded calcium indicators (GECIs) have expanded the available pallet of colors used for the visualization of neuronal calcium activity in vivo. However, their calcium-binding domain is restricted by calmodulin from metazoans. In this study, we developed red GECI, called FRCaMP, using calmodulin (CaM) from

Indexed as

CalmodulinGreen Fluorescent ProteinsRecombinant Fusion ProteinsSchizosaccharomyces pombe ProteinsAnimalsCalciumHeLa CellsHumansMiceNeuronsSchizosaccharomycesCalciumCalmodulinGreen Fluorescent ProteinsRecombinant Fusion ProteinsSchizosaccharomyces pombe ProteinsbJun/bFoscalcium imagingfluorescent proteinFRCaMPGECIgenetically encoded calcium indicatorprotein engineeringred fluorescentSchizosaccharomyces pombesplit

Identifiers

PMID33374320
PMCPMC7794825
OpenAlexW3113946345

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.