ArticleMicrobiologyOpen2021
Effect of storage, temperature, and extraction kit on the phylogenetic composition detected in the human milk microbiota.
Article in MicrobiologyOpen, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Distinct associations among human milk microbiota and minerals in relation to early infant growth: a longitudinal study.Journal of translational medicine · 2026Article
- Characterizing Human Milk RNA Degradation Over Time to Optimize Storage of Human Milk for RNA Quality.Journal of mammary gland biology and neoplasia · 2026Article
- The Microbial Composition of Bovine Colostrum as Influenced by Antibiotic Treatment.Antibiotics (Basel, Switzerland) · 2025Article
- Milk mineral composition is strongly associated with the human milk microbiome.Frontiers in nutrition · 2025Article
- Sequencing the ocular surface microbiome: a review of methodological practices and considerations.Frontiers in ophthalmology · 2025Review
- Comparison of canine colostrum and milk using a multi-omics approach.Animal microbiome · 2024Article
- Human milk microbiome: associations with maternal diet and infant growth.Frontiers in nutrition · 2024Article
- Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniques.ISME communications · 2023Article
- Improvement and Validation of a Genomic DNA Extraction Method for Human Breastmilk.Methods and protocols · 2023Article
- Experiences of mothers of NICU preterm infants in milk management out of the hospital: a qualitative study.International breastfeeding journal · 2022Article
- Comparison of the Effectiveness of Four Commercial DNA Extraction Kits on Fresh and Frozen Human Milk Samples.Methods and protocols · 2022Article
- The human milk microbiome aligns with lactation stage and not birth mode.Scientific reports · 2022Article
- Human milk microbiome is shaped by breastfeeding practices.Frontiers in microbiology · 2022Article
- A culture-, amplification-independent, and rapid method for identification of pathogens and antibiotic resistance profile in bovine mastitis milk.Frontiers in microbiology · 2022Article
- Contrasting Diversity and Composition of Human Colostrum Microbiota in a Maternal Cohort With Different Ethnic Origins but Shared Physical Geography (Island Scale).Frontiers in microbiology · 2022Article
- Oligosaccharides and Microbiota in Human Milk Are Interrelated at 3 Months Postpartum in a Cohort of Women with a High Prevalence of Gestational Impaired Glucose Tolerance.The Journal of nutrition · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human milk is considered the optimum feeding regime for newborns and is a source of bacteria for the developing infant gastrointestinal tract. However, as with all low biomass samples, standardization across variabilities such as sample collection, storage, and extraction methods is needed to eliminate discrepancies in microbial composition across studies. The aim of this study was to investigate how different storage methods, temperatures, preservatives, and extraction kits influence the human milk microbiome, compared to fresh samples. Breast milk samples were processed via six different methods: fresh (Method 1), frozen at -80°C (Method 2), treated with RNAlater and stored at 4°C or -80°C (Methods 3 and 4), and treated with Milk Preservation Solution at room temperature (Methods 5 and 6). Methods 1-5 were extracted using PowerFood
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